Cells were plated and incubated overnight before being treated, in duplicate, with inhibitors or vehicle control in phenol red–free RPMI-1640 without FBS for 8 hours at 37°C. Cells were placed on ice and rinsed with cold 1× PBS. Cells were collected and RNA was isolated using the Qiashredder (Qiagen) and RNeasy Mini Kit (Qiagen). RNA was quantified on the NanoDrop 2000 spectrophotometer (Thermo Scientific) and RNA quality was inspected on a 1% agarose gel. Biotin labeled RNA was hybridized to Illumina 3′IVT human HT-12 BeadChip arrays.
3 ivt human ht 12 beadchip arrays
Manufactured by Illumina
The 3′IVT human HT-12 BeadChip arrays are a type of high-throughput gene expression microarray produced by Illumina. The arrays contain probes designed to target over 47,000 human transcripts, allowing for the comprehensive analysis of the human transcriptome. The arrays utilize Illumina's proprietary bead-based technology to provide accurate and sensitive gene expression data.
Automatically generated - may contain errors
2 protocols using 3 ivt human ht 12 beadchip arrays
1
RNA Extraction and Microarray Analysis
2
Microarray Expression Profiling of RNA
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Cells were collected and RNA was isolated using the Qiashredder (Qiagen) and RNeasy Mini Kit (Qiagen). RNA was quantified on the NanoDrop 2000 spectrophotometer (Thermo Scientific) and RNA quality was inspected on a 1% agarose gel. Biotin labeled RNA was hybridized to Illumina 3′IVT human HT-12 BeadChip arrays.
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