After dissection, the liver was placed in 10% buffered formalin and later embedded in paraffin blocks. The paraffin blocks were then attached to a microtome (Leitz-1512 Microtome, Leitz, Wetzlar, Germany) for cutting. The slides were then stained with hematoxylin-eosin and washed in running water. In the dehydration phase, the structures went through a series of three baths: One in absolute alcohol and two in xylol. The cover slip was then fixed into place using Canada balsam or Entellan, which completed the preparation process. The slides were analyzed with a Nikon Labophot binocular microscope equipped with a digital camera. Using the Image-Plus software (Media Cybernetics, Bethesda, MD, United States), images were captured at different magnifications.
Labophot binocular microscope
Manufactured by Nikon
The Labophot binocular microscope is a high-quality laboratory equipment designed for various scientific and research applications. It features binocular eyepieces, allowing users to observe specimens with both eyes. The microscope provides magnification capabilities, enabling detailed examination and analysis of samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using labophot binocular microscope
1
Histological Analysis of Liver Samples
2
Histopathological and Immunohistochemical Analysis of Colon Inflammation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After the death of the animals, their colons were removed and fixed in 10% buffered formalin for 24 h. Paraffin blocks were sliced with a rotary microtome to create 3 mm thick sections. The tissues were stained with hematoxylin and eosin. The slides were photographed using a NIKON Labophot binocular microscope at a magnification of 200×. The histological analysis was based on changes in the crypts and the presence of inflammation in the colon [26 (link)]. The expression of COX-2 and TNF-α proteins in intestinal tissues was determined by immunohistochemical analysis, as described by Hartmann et al. [26 (link)]. The slides were analyzed using a microscope equipped with a digital camera using Image-Plus software (Media Cybernetics, Bethesda, MD, USA). The quantification of COX-2 and TNF-α expression was performed via digital analysis with ImageJ and involved counting the positive pixels stained by immunohistochemical analysis with the IHC profiler plugin.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!