Mci buffer l 8800 ph 1 4

Ficoll-Paque was obtained from Pharmacia (Uppsala, Sweden). Fibronectin was from Calbiochem (La Jolla, San Diego, CA, USA). Bicarbonate-free Hank’s solution, Ca2⁺-free Dulbecco PBS, cytochalasin D, minoxidil, doxycycline, wortmannin, Akt 1/2 inhibitor, cytochalasin D, latrunculin A, blebbistatin, staurosporine, 4-bromophenacyl bromide, and E64 were obtained from Sigma (Steinheim, Germany). Analytical chromatography conditions: eluent MCI Buffer L-8800-PH-1–4 and ninhydrin coloring solution kit for Hitachi 29,970,501 (Wako Chemicals, North Chesterfield, VA, USA). Coomassie Brilliant Blue G-250 was obtained from Serva, PMSF from MP Biomedical, trypan blue from Fluka AG, trypsin from Promega, glutaraldehyde from Ted Pella, carboxy-H₂DCF-DA from Molecular Probe, (Eugene, OR, USA).

Bicarbonate-free Hank’s solution, Ca2⁺-free Dulbecco PBS, imipramine, LPS (lipopolysaccharide from Salmonella enterica serovar Typhimurium) and E64 were obtained from Sigma (Steinheim, Germany). Ficoll-Paque was obtained from Pharmacia (Uppsala, Sweden). Fibronectin was from Calbiochem (La Jolla, United States). Coomassie Brilliant Blue G-250 was obtained from Serva, PMSF from MP Biomedical, trypan blue from Fluka AG, glutaraldehyde from Ted Pella. Trypsin was from Promega, carboxy-H₂DCF-DA from Molecular probe, United States. Analytical chromatography conditions: eluent MCI Buffer L-8800-PH-1–4 and ninhydrin coloring solution kit for Hitachi 29970501 (Wako Chemicals GmbH, United States).