Goat anti rabbit dylight 550

Manufactured by Thermo Fisher Scientific

Sourced in Canada, United States

Goat anti-Rabbit DyLight 550 is a secondary antibody that binds to rabbit primary antibodies. It is conjugated with the DyLight 550 fluorescent dye, which can be detected using appropriate instrumentation.

Automatically generated - may contain errors

Lab products found in correlation

Mouse anti stat3, by Cell Signaling Technology (1 mentions) Goat anti mouse dylight 633, by Thermo Fisher Scientific (1 mentions) Anti stat3, by BD (1 mentions) Bio beads sm 2 adsorbants, by Bio-Rad (1 mentions) Arachidonic acid d8, by Cayman Chemical (1 mentions) 2 arachidonyl glycerol d5, by Cayman Chemical (1 mentions) Recombinant m csf, by Thermo Fisher Scientific (1 mentions) Triarachidonin, by Nu-Chek Prep (1 mentions) Kt109, by Cayman Chemical (1 mentions) Rat anti mouse f4 80 antibody, by Bio-Rad (1 mentions) 2 mercaptoethanol, by Merck Group (1 mentions) Polyethyleneimine, by Polysciences (1 mentions) Phospho p44 42 mapk erk1 2 thr202 tyr204 antibody, by Cell Signaling Technology (1 mentions) Phospho p90rsk thr359 ser363 antibody, by Cell Signaling Technology (1 mentions) Ulixertinib bvd 523, by Selleck Chemicals (1 mentions) Vx 11e, by Selleck Chemicals (1 mentions) Fetal bovine serum (fbs), by Omega Scientific (1 mentions) Mouse anti α tubulin, by Abcam (1 mentions) Alexa fluor 488 phalloidin, by Cell Signaling Technology (1 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions)

4 protocols using goat anti rabbit dylight 550

STAT3 Activation Profiling by Flow Cytometry and Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies used for flow cytometry: Alexa Fluor 488 Mouse anti-STAT3 (pY705) (Clone 4/P-STAT3), BD Phosflow, Franklin Lakes, NJ, USA, Catalog No. 557814, Lot: 4003621; PE Mouse anti-STAT3 (Clone M59-50), BD Phosflow, Catalog No. 560391, Lot: 7046704; Mouse anti-gp130 (Clone BR-3), Hölzel Diagnostics, Cologne, Germany, Catalog No. 852.060.000, Lot:P11016D6.
Antibodies used for immunodetection of Western Blots: Rabbit anti-STAT3 (pY705) (Clone D3A7), Cell Signaling Technology, Cambridge, UK, Catalog No. 9145, Lot: 34; Rabbit anti-STAT3 (pS727) (Polyclonal), Cell Signaling Technology, Catalog No. 9134, Lot: 21; Mouse anti-STAT3 (Clone 124H6), Cell Signaling Technology, Catalog No. 9139, Lot: 10; Rabbit anti-SOCS3 (Clone C204), Immuno-Biological Laboratories, Fujioka, Japan, Catalog No. 18391, Lot: 0G-901; Mouse anti-HSC70 (Clone 1F2-H5), StressMarq Biocsciences, Victoria, Canada, Catalog No. SMC-151, Lot: 0706; Goat anti-Mouse DyLight 633 (polyclonal), Thermo Fisher Scientific, Catalog No. 815-968-0747, Lot: NB167398; Goat anti-Rabbit DyLight 550 (polyclonal), Thermo Fisher Scientific, Catalog No. 815-968-0747, Lot: NB165012.

Billing U., Jetka T., Nortmann L., Wundrack N., Komorowski M., Waldherr S., Schaper F, & Dittrich A. (2019). Robustness and Information Transfer within IL-6-induced JAK/STAT Signalling. Communications Biology, 2, 27.

+ Open protocol

+ Expand

Macrophage Lipid Characterization Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Unless otherwise specified, reagents used were purchased from Fisher Scientific. Bio-beads SM-2 Adsorbants (Bio-Rad) catalog# 1528920. Polyethyleneimine (Polysciences, Inc.) catalog# 24765, 2-Mercaptoethanol (Sigma-Aldrich) catalog# M6250–250ML, recombinant M-CSF (Peprotech) catalog# 315–02. KT109 (synthesized in house), KT195 (Cayman Chemical Company) catalog# 14818. Anti-FLAG antibody produced in rabbit (Sigma-Aldrich) catalog# F7425, Goat anti-rabbit DyLight 550 (Thermo Fisher Scientific) catalog# 84541. PE/Cy7 anti-mouse/human CD11b antibody (Biolegend) catalog# 101215. Rat anti-mouse F4/80 antibody (Bio-Rad) catalog# MCA497A488T. Arachidonic acid-d8 (Cayman Chemical Company) catalog# 390010, 2-Arachidonyl glycerol-d5 (Cayman Chemical Company) catalog# 362162, Tritetradecanoin (C14:0) (Nu-Chek Prep) catalog# T-140, Trihexadecanoin (C16:0) (Nu-Chek Prep) catalog# T-150, Trioctadecanoin (C18:0) (Nu-Chek Prep) catalog# T-160, Trieicosanoin (C20:0) (Nu-Chek Prep) catalog# T-170, Tridocosanoin (C22:0) (Nu-Chek Prep) catalog# T-180, Triarachidonin (C20:4) (Nu-Chek Prep) catalog# T-295, Trieicosapentaenoin (C20:5) (Nu-Chek Prep) catalog# T-325, Tridocosahexaenoin (C22:6) (Nu-Chek Prep) catalog# T-310. Fetal bovine serum (FBS) and dialyzed FBS were obtained from Omega Scientific.

Shin M., Ware T.B, & Hsu K.L. (2020). DAGL-beta functions as a PUFA-specific triacylglycerol lipase in macrophages. Cell chemical biology, 27(3), 314-321.e5.

+ Open protocol

+ Expand

Evaluating MAPK/ERK Pathway Inhibitors

Check if the same lab product or an alternative is used in the 5 most similar protocols

Reagents used were purchased from Fisher Scientific unless specified otherwise. Primary antibodies were purchased from Cell-Signaling Technologies: phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody (catalog # 9101S); phospho-p90RSK (Thr359/Ser363) Antibody (catalog # 9344S). Secondary fluorescent conjugated antibodies were obtained from Thermo Fisher Scientific (goat anti-Rabbit-DyLight 550, catalog # 84541). Fetal bovine serum (FBS), and dialyzed fetal bovine serum (dialyzed-FBS) were obtained from Omega Scientific. Ulixertinib (BVD-523, catalog # S7854) and VX-11e (catalog # S7709) were obtained from Selleckchem.

Shin M., Franks C.E, & Hsu K.L. (2018). Isoform-selective activity-based profiling of ERK signaling †Electronic supplementary information (ESI) available: Experimental Methods and Supplementary Figures. See DOI: 10.1039/c8sc00043c. Chemical Science, 9(9), 2419-2431.

+ Open protocol

+ Expand

Zinc Oxide Nanoparticles Cytotoxicity Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The TM4 cells were incubated with different concentrations of ZnO NPs (0, 0.2, 0.5, 0.75, 1.0, and 1.25 µg/mL) for 24 and 48 hours. Then, cells were fixed with 4% paraformaldehyde for 20 minutes at room temperature. Fixed cells were blocked with 0.1% Triton X-100 (Merck, USA) and 3% BSA in PBS for 30 minutes after permeabilizing by 0.1% Triton X-100 in Triton/PBS solution for 15 minutes. Subsequently, cells were incubated with rabbit anti-LC3B (1:100) (Thermo Fisher Scientific, USA), mouse anti-Arp3 (1:50) (Sigma-Aldrich, USA), mouse anti-α-tubulin (1:300) (Abcam, USA), and rabbit γ-H2AX (1:200) (Millipore-Sigma, Burlington, MA, USA) in 0.1% TX-100/PBS overnight at 4 °C. After washing twice with 0.05% Tween-20/PBS, cells were incubated with goat anti-mouse Dylight 650 (1:1,000) (Thermo Fisher Scientific, USA), goat anti-rabbit Dylight 550 (1:1,000) (Thermo Fisher Scientific, USA) and the DNA staining dye Hoechst 33342 (Molecular Probes, Eugene, OR, USA) in PBS/BSA for 90 minutes at room temperature in the dark, followed by incubation with 50 µL Alexa Fluor 488 Phalloidin (Cell Signaling Technology, Danvers, MA, USA) per well at room temperature for 30 minutes for F-actin staining.

Hong X., Shao N., Yin L., Li C., Tao G., Sun Y., Qian K., Yang J., Xiao P., Yu X, & Zhou Z. (2022). Exposure to zinc oxide nanoparticles affects testicular structure, reproductive development and spermatogenesis in parental and offspring male rats. Annals of Translational Medicine, 10(13), 751.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!