DUX4-FL was detected with rabbit anti-DUX4-FL mAb E5524 (link) used at 1:200 dilution (Epitomics, Burlingame, CA). Myosin heavy chain (MyHC) isoforms were detected with mouse mAbs F59 or MF20 (Developmental Studies Hybridoma Bank, Iowa City, IA) used at 1:10 dilution of hybridoma supernatant. Activated caspase-3 was detected with a rabbit pAb (Cell Signaling Technologies, Beverly, MA) used at 1:100. TDP-43 was detected with either rabbit anti-TARDBP pAb (cat. 10782-2-AP; Proteintech, Chicago, IL) or mouse anti-TDP-43 mAb (cat. 60019-2; Proteintech). Ubiquitinated proteins were detected with mouse mAb FK2 (MBL, Woburn, MA) which reacts with K29-, K48-, and K63 mono- and poly-ubiquitinated proteins, but not free ubiquitin. V5 epitope tag was detected using either mouse anti-V5 mAb (Life Technologies, Grand Island, NY) or a rabbit pAb (EMD Millipore). GFP was detected with a rabbit pAb (Santa Cruz Biotechnology, Dallas, TX). Primary antibody binding was visualized with appropriate species-specific secondary antibodies (Life Technologies) conjugated to either Alexa Fluor 488 or Alexa Fluor 594 and used at 1:500. Nuclei were stained with bisbenzimide.
Rabbit pab
Manufactured by Merck Group
Rabbit pAb is a polyclonal antibody raised in rabbits. It is used as a general-purpose tool in various laboratory applications.
Automatically generated - may contain errors
2 protocols using rabbit pab
1
Immunofluorescent Labeling of Protein Markers
2
CGRP-Positive Nerve Staining in Bladder
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Nerves were stained with CGRP (Anti-Calcitonin Gene Related Peptide), Rabbit pAB (Merck) with Alexa 594 secondary antibody (Life Technologies TM ). Fixed bladder tissue was washed 3 times with Tris-Buffered Saline (TBS) (Merck), each for 15 min. Primary antibody (CGRP) was diluted 1:1000 in TBS-Triton (0,3%), applied to the tissue, and incubated overnight at 4 C. The primary antibody was removed and the sample was washed 3 times, each for 30 min with TBS-Triton (0,3%), TBS and TBS-Triton (0,3%), respectively. Secondary antibody (Alexa 594) was diluted 1:100 in TBS-Triton (0,3%), applied to the sample and incubated in the dark, overnight at 4 C. The secondary antibody was removed and the sample was washed 3 times, each for 15 min, with TBS-Triton (0,3%), TBS and TBS, respectively
During TPLSM imaging the prepared bladders were placed in a petri dish and submerged under saline solution (Schueth et al., 2014) . Images were taken from the urothelial side. In each bladder, both dome and trigone regions were imaged.
During TPLSM imaging the prepared bladders were placed in a petri dish and submerged under saline solution (Schueth et al., 2014) . Images were taken from the urothelial side. In each bladder, both dome and trigone regions were imaged.
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