Fitc conjugated anti mouse cd11b clone m1 70

Manufactured by BioLegend

FITC-conjugated anti-mouse CD11b (clone M1/70) is a monoclonal antibody that binds to the CD11b antigen expressed on the surface of mouse myeloid cells, including monocytes, macrophages, and granulocytes. The antibody is conjugated to the fluorescent dye FITC, enabling the detection and analysis of CD11b-positive cells using flow cytometry.

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Lab products found in correlation

Efluor 780 conjugated fixable viability dye, by Thermo Fisher Scientific (1 mentions) Gallios flow cytometer, by Beckman Coulter (1 mentions) Percp efluor 710 conjugated anti mouse cd3 clone 17 a2, by Thermo Fisher Scientific (1 mentions) Pa3 900, by Thermo Fisher Scientific (1 mentions) Alexafluor 488 goat anti rabbit igg a 11008, by Thermo Fisher Scientific (1 mentions) Fcr blocker, by Miltenyi Biotec (1 mentions) Navios flow cytometer, by Beckman Coulter (1 mentions)

3 protocols using fitc conjugated anti mouse cd11b clone m1 70

Multiparametric Flow Cytometry of Macrophages

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Cells were incubated with anti-mouse CD16/32 (Fc receptor block, clone 93; Biolegend, San Diego, CA) and then with FITC-conjugated anti-mouse CD11b (clone M1/70; Biolegend), PE-conjugated anti-mouse Ly6C (clone HK1.4; Biolegend), PE/Cy7-conjugated anti-mouse F4/80 (clone BM8; Biolegend), and AF 647-conjugated anti-mouse Ly6G (clone 1A8; Biolegend) antibodies or appropriate isotypic controls for 30 min at 4 °C. This was followed by incubation with eFluor 780-conjugated fixable viability dye (eBioscience, San Diego, CA). Cells were analyzed on a Beckman Coulter Gallios flow cytometer (Brea, CA); data were analyzed using Kaluza version 1.2 software. Macrophages were separated from contaminating endothelial cells on the basis of forward and side scatter, and on expression of CD11b. Viable CD11b⁺ macrophages were then analyzed for expression of Ly6G. This was followed by analysis of Ly6C and then F4/80 (Fig. 1).

Mandal M., Gardner C.R., Sun R., Choi H., Lad S., Mishin V., Laskin J.D, & Laskin D.L. (2016). The spleen as an extramedullary source of inflammatory cells responding to acetaminophen-induced liver injury. Toxicology and applied pharmacology, 304, 110-120.

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Multicolor Flow Cytometry Antibody Panel

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Rabbit polyclonal antibody against calreticulin (PA3-900) was purchased from ThermoFisher (Rockford, IL, USA). Alexa Fluor 488 goat anti-rabbit IgG (A11008) was purchased from Life Technology (Eugene, OR, USA). PE-conjugated anti-mouse CD4 (clone RM4-5),PE/Cy7-conjugated anti-mouse CD8 (clone 53-6.7), FITC-conjugated anti-mouse CD25 (clone 3C7), Alexa Fluor 488-conjugated anti-mouseFoxP3 (clone 150D), APC-conjugated anti-mouse Gr-1 (clone RB6-8C5) and FITC-conjugated anti-mouse CD11b (clone M1/70) antibodies were purchased from BioLegend (San Diego, CA). PerCP-eFluor 710-conjugated anti-mouse CD3 (clone 17-A2)and APC eFluor 780-conjugatedanti-mouse CD127 (clone A7R34) antibodies were purchased from eBioscience (San Diego, CA).

Tagliamonte M., Petrizzo A., Napolitano M., Luciano A., Rea D., Barbieri A., Arra C., Maiolino P., Tornesello M., Ciliberto G., Buonaguro F.M, & Buonaguro L. (2016). A novel multi-drug metronomic chemotherapy significantly delays tumor growth in mice. Journal of Translational Medicine, 14, 58.

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Isolation and Analysis of Hepatic MNCs

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Hepatic MNCs were isolated from the mice at 3 h and 8 h after Con-A treatment, and incubated with FCR blocker (cat: 130-092-575, Miltenyi Biotec) for 20 min in order to prevent nonspecific binding. The cells were stained with various antibodies, including fluorescein isothiocyanate (FITC)- conjugated anti-mouse CD11b (clone M1/70, cat: 101206, BioLegend), APC-conjugated anti-mouse F4/80 (clone BM8, cat: 123115, BioLegend). Cells were detected and analyzed with Beckman Coulter Navios Flow Cytometer and the Kaluza.

Sun X., Ni Y., Lu Q., Liang Y., Gu M., Xue X, & Dai C. (2022). Mechanistic target of rapamycin complex 1 orchestrates the interplay between hepatocytes and Kupffer cells to determine the outcome of immune-mediated hepatitis. Cell Death & Disease, 13(12), 1031.

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