Apc anti mouse human cd45r b220

Manufactured by BioLegend

Sourced in United States

The APC anti-mouse/human CD45R/B220 is a fluorophore-conjugated antibody that binds to the CD45R/B220 antigen, which is expressed on the surface of B cells and some other hematopoietic cells. This product is suitable for flow cytometry applications.

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Anti-B220 (40 citations) B220-APC (23 citations) CD45R/B220 (19 citations) Anti-B220-APC- (9 citations) Anti-B220 (CD45R) (5 citations) APC-CD45R/B220 (4 citations) APC anti-mouse B220 (4 citations) Anti-mouse B220 (4 citations) Anti-CD45R (4 citations) Anti-mouse CD45R-APC (2 citations)

3 protocols using apc anti mouse human cd45r b220

Multiparameter Flow Cytometry Assay

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For surface markers, the cells were stained in PBS containing 1% BSA with indicated antibodies for 30 min on ice. For intracellular markers, the cells were first fixed with Fixation Buffer (420801; Biolegend, San Diego, CA, USA) at 4 °C for 30 min and then stained in Permeabilization Wash Buffer (421002; Biolegend, San Diego, CA, USA) with relevant antibodies at 4 °C for 30 min. Foxp3 staining was conducted according to the manufacturer’s instructions for the Mouse Foxp3 Buffer Set obtained from BD Bioscience (San Diego, CA, USA). The following antibodies were used for the studies: APC anti-mouse CD45 (103112), PE anti-mouse F4/80 (123110), PerCP/Cy5.5 anti-mouse F4/80 (123128), FITC anti-mouse CD11c (117306), APC anti-mouse CD206 (141708), APC anti-mouse/human CD45R/B220 (103211), PerCP/Cy5.5 anti-mouse Ly-6G/Ly-6C (108427), FITC anti-mouse CD4 (100406), PerCP anti-mouse CD8a (100732), AlexaFluor 647 anti-mouse/rat/human Foxp3 (320014), PE anti-mouse/human CD44 (103008), and APC anti-mouse CD62L (104412) from Biolegend (San Diego, CA, USA), and PE-Cy7 anti-mouse CD11b (552850) from BD Bioscience (San Diego, CA, USA). Flow cytometry data were acquired on MACSQuant^TM (Miltenyi Biotec, Auburn, CA, USA) and analyzed by FlowJo software (v10.5.3).

Chen L., Zhang J., Zou Y., Wang F., Li J., Sun F., Luo X., Zhang M., Guo Y., Yu Q., Yang P., Zhou Q., Chen Z., Zhang H., Gong Q., Zhao J., Eizirik D.L., Zhou Z., Xiong F., Zhang S, & Wang C.Y. (2021). Kdm2a deficiency in macrophages enhances thermogenesis to protect mice against HFD-induced obesity by enhancing H3K36me2 at the Pparg locus. Cell Death and Differentiation, 28(6), 1880-1899.

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Immune Cell Dynamics in RBMRNA-405 Treatment

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RBMRNA-405-induced effects on proliferation and dynamics of immune cell populations were evaluated using Accuri C6 (BD Biosciences). For T-cell subset analysis, 1×10⁶ mice splenocytes (100 μl) were seeded per well into 96-well plates. Following two washes with PBS, the viability of cells was determined using Zombie Green™ Fixable Viability Kits (BioLegend, 423111). After washing once with FC buffer (PBS + 2% FBS), cells were resuspended in FC buffer. Then, splenocytes were stained with PerCP/Cyanine5.5 anti-mouse CD3 (BioLegend, 100218), PE anti-mouse CD8a (BioLegend, 100708), APC anti-mouse CD4 (BioLegend, 100412), PE anti-mouse CD62L (BioLegend, 161203), and APC anti-mouse/human CD44 (BioLegend, 103011). For GC response analysis, inguinal and iliac lymph nodes (LNs) were pooled and lymphocytes were isolated. Cells were treated with the same method as splenocytes except for the following antibodies: APC anti-mouse CD4 (BioLegend, 100412), PE anti-mouse CD185 (CXCR5) (BioLegend, 145503), PerCP/Cyanine5.5 anti-mouse CD278 (ICOS) (BioLegend, 117424), APC anti-mouse CD19 (BioLegend, 115512), PE anti-mouse CD95 (Fas) (BioLegend, 152608), PE anti-mouse CD138 (Syndecan-1) (BioLegend, 142504), APC anti-mouse/human CD45R/B220 (BioLegend, 103212), and PE anti-mouse CD79b (Igβ) (BioLegend, 132804).

Ma Q., Li M., Ma L., Zhang C., Zhang H., Zhong H., Wen J., Wang Y., Yan Z., Xiong W., Wu L., Guo J., Yang W., Yang Z, & Zhang B. (2023). SARS-CoV-2 bivalent mRNA vaccine with broad protection against variants of concern. Frontiers in Immunology, 14, 1195299.

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Comprehensive Immune Cell Analysis by Flow Cytometry

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To analyse DCs by flow cytometry, CNS, lymph nodes and splenic cell suspensions were incubated with surface antibodies and a live–dead cell marker on ice. After 30 min, cells were washed with 0.5% BSA and 2 mM EDTA in 1× PBS, and fixed according to the manufacturer’s protocol (eBiosciences, 00–5523-00). Intracellular staining was performed for 1 h at room temperature. The surface antibodies used in this study were: BUV395 anti-mouse MHC-II (17–5321-82, Invitrogen, 1:200), BUV496 anti-mouse CD24 (564664, BD Biosciences, 1:100), BUV563 anti-mouse Ly6G (612921, BD Biosciences, 1:100), BUV661 anti-mouse CD45 (103147, BioLegend, 1:100), BV570 anti-mouse Ly6C (128030, BioLegend, 1:100), BV605 anti-mouse CD80 (563052, BD Biosciences, 1:100), BV650 anti-mouse CD3 (100229, BioLegend, 1:100), BV711 anti-mouse CD8a (100748, BioLegend, 1:100), BV786 anti-mouse CD11b (101243, BioLegend, 1:100), PE-Texas Red anti-mouse CD11c (117348, BioLegend, 1:100), PE-Cy7 anti-mouse CD4 (100422, BioLegend, 1:100), APC anti-mouse/human CD45R/B220 (103212, BioLegend, 1:100), APC-R700 anti-mouse CD103 (565529, BD Biosciences, 1:100) and APC/Cy7 anti-mouse F4/80 (123118, BioLegend, 1:100). The intracellular antibody used was Alexa Fluor 488 anti-mouse HIF-1α (BS-0737R-A488, Bioss Antibodies, 1:100). Fluorescence-activated cell sorting (FACS) was performed on a Symphony A5 (BD Biosciences).

Zhu Y., Traub L.M, & Kornfeld S. (1999). High-Affinity Binding Of The AP-1 Adaptor Complex to Trans-Golgi Network Membranes Devoid Of Mannose 6-Phosphate Receptors. Molecular Biology of the Cell, 10(3), 537-549.

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