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Monoclonal anti α tubulin

Manufactured by Beyotime
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Sourced in United States, China

Monoclonal anti-α-tubulin is a laboratory reagent used for the detection and analysis of α-tubulin, a key component of the cytoskeleton in eukaryotic cells. This antibody specifically binds to α-tubulin, allowing researchers to visualize and study its distribution and dynamics within cells.

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Lab products found in correlation

Anti β actin, by Beyotime (2 mentions) Monoclonal anti ub, by Santa Cruz Biotechnology (2 mentions) Anti fancd2, by Abcam (1 mentions) Anti erk, by Cell Signaling Technology (1 mentions) Anti egfr, by Cell Signaling Technology (1 mentions) Anti lamp2, by Abcam (1 mentions) Anti her3, by Cell Signaling Technology (1 mentions) Anti her2, by Santa Cruz Biotechnology (1 mentions) Anti her2, by Abcam (1 mentions) Monoclonal anti perk, by Cell Signaling Technology (1 mentions) Anti caspase 3, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

α-Tubulin (28 citations) Anti-α-tubulin (17 citations) Anti-tubulin (15 citations)

2 protocols using monoclonal anti α tubulin

1

Detailed Western Blot Analysis Protocol

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Western blot analyses were performed as previously described.41 (link) The antibodies used were as follows: monoclonal anti-α-tubulin, anti-β-actin (1 : 2000, Beyotime); monoclonal anti-XRCC1, anti-CTR1, anti-MRP2, anti-DAN-PK, anti-FANCD2 (1 : 2000, Epitomics, Burlingame, CA, USA); polyclonal anti-TXNL1 (1 : 2000, Proteintech, Chicago, IL, USA); anti-γH2AX (1 : 2000, Upstate, New York, NY, USA); monoclonal anti-PARP (1 : 1000, Cell Signaling Technology, Danvers, MA, USA); and monoclonal anti-Ub (1 : 500, Santa Cruz, Dallas, TX, USA). CHX and MG132 were purchased from Sigma-Aldrich.
Xu W., Wang S., Chen Q., Zhang Y., Ni P., Wu X., Zhang J., Qiang F., Li A., Røe O.D., Xu S., Wang M., Zhang R, & Zhou J. (2014). TXNL1-XRCC1 pathway regulates cisplatin-induced cell death and contributes to resistance in human gastric cancer. Cell Death & Disease, 5(2), e1055-.
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2

Western Blot Analysis of Signaling Proteins

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Western blotting was performed as previously described [31 (link)]. The antibodies used were as follows: monoclonal anti-JWA (1 : 500, contract produced by AbMax, Beijing, China); monoclonal anti-α-tubulin, anti-β-actin (loading control) (1 : 2000, Beyotime, Haimen, Jiangsu, China); monoclonal anti-P-ERK, anti-ERK, anti-P-AKT(473), anti-AKT, anti-Caspase 3, anti-EGFR, anti-HER3 (1 : 1000, Cell Signaling Technology, Danvers, MA, USA); monoclonal anti-Ub (1 : 500) and polyclonal anti-HER2 (1 : 1000) (Santa Cruz, Dallas, TX, USA); monoclonal anti-c-Cbl (1 : 2000), anti-Lamp2 and anti-HER2 (1 : 250, Abcam, USA).
Ma L., Zhu W., Wang Q., Yang F., Qian J., Xu T., Wang S., Zhou J, & Shu Y. (2016). JWA down-regulates HER2 expression via c-Cbl and induces lapatinib resistance in human gastric cancer cells. Oncotarget, 7(44), 71790-71801.
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