Md 2010

C. asiatica (1 g) was extracted with 10 mL of 20 % ethanol at 37 °C for 24 h. The extract was filtered with a 0.45-μm filter and analyzed by HPLC. HPLC analyses were performed on a LC-2000 series apparatus (Jasco) with a PU-2089 plus pump and a MD-2010 plus diode array detector, equipped with a LUNA C18 column (250 × 4.6 mm inner diameter; 5-μm particle size; Phenomenex, Torrance, CA, USA). The wavelength of diode array detector was set at 206 nm. The analytical method was based on previous study.¹⁷

The RMD powder (1 g) was extracted with 10 mL of methanol at 60°C for 30 minutes. The extract was further filtered with a 0.45-μm filter and analyzed using high-performance liquid chromatography. High-performance liquid chromatography analyses were performed on a LC-2000 series (Jasco, Tokyo, Japan) apparatus with a PU-2089 plus pump and an MD-2010 plus diode array detector, equipped with a LUNA C₁₈ column (250 × 4.6 mm inner diameter, 5 μm particle size; Phenomenex, Torrance, CA, USA). Wavelength of the diode array detector was set at 238 nm. The analytical method was based on our previous study [14 (link)].