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2 protocols using isoamyl propionate

1

Analytical Standards for Aroma Compounds

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Dichloromethane, ethyl acetate, absolute ethanol (99%), and anhydrous sodium sulfate were purchased from VWR International (Leuven, Belgium). Standards of ethyl benzoate, methyl anthranilate, phenylacetaldehyde, 2-methylbutanal, 3-methylbutanal, heptan-2-one, heptan-2-ol, 2-methylbutyl acetate, trans-2-nonenal, methional, isobutyl acetate, benzaldehyde, myrcene, linalool, ethyl heptanoate, ethyl octanoate, 2-phenylethyl acetate, ethyl acetate, ethyl butanoate, isoamyl propionate, acetophenone, and 2-acetylthiophene were purchased from Sigma-Aldrich (Bornem, Belgium). Standard of nonan-2-one was purchased from Janssen Chimica (Geel, Belgium). Standards of dimethyltrisulfide and 1-octen-3-ol were purchased from Acros Organics (Geel, Belgium). Standard of 3-methylbutan-1-ol was purchased from Merck Millipore (Burlington, NJ, USA). Standard of acetoin was purchased from Supelco (Bellefonte, PA, USA).
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2

Formulation and Evaluation of Varnish

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The extract was placed in a Branson Bath® Sonicator 1800 (Branson Ultrasonics, Danbury, CT) along with ethyl acetate (Sigma-Aldrich® Chemicals Pvt. Ltd., Bangalore, India) for about 30 minutes for the complete dissolution of the extract. After its dissolution, isoamyl propionate (Sigma-Aldrich® Chemicals Pvt. Ltd., Bangalore, India) and the colloidal solution (Vivid India® Chemicals Pvt. Ltd., New Delhi, India) were added. The contents were mixed in a MixMate® Vortex Agitator (Eppendorf, Sydney, Australia) at 1000 rpm (maximum setting) for about 30 seconds, and fumed silica (Research-Lab Fine Chem Industries, Mumbai, India) was added. The contents were then transferred to an amber-coloured sterile bottle and labelled. Physical properties of varnish such as colour matching, rate of evaporation, viscosity, pH of the varnish and film-forming ability were evaluated.
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