Murine il 17a elisa kits

To investigate the immunomodulatory effects of AMM/B on synovial fibroblast, 5 × 10⁵ synovial fibroblasts were treated without or with 10 ng/mL tumor necrosis factor (TNF)-α for 24 h and then co-cultured with 5 × 10⁵ AMMs or AMM/B in RPMI 1640 containing 10% FBS. The co-cultured supernatants were harvested after two days, and the IL34 cytokine levels were evaluated using a murine IL-34 ELISA kit (R&D Systems, Minneapolis, MN, USA). The concentration of IL-17A was measured using murine IL-17A ELISA kits (R&D Systems) and mouse serum. The BMP-2 protein level was also examined using a human BMP-2 ELISA kit (R&D Systems).