Fshr antibody

Manufactured by Proteintech

The FSHR antibody is a laboratory reagent used for the detection and analysis of the follicle-stimulating hormone receptor (FSHR) protein. The FSHR protein is a key component in the regulation of reproductive functions.

Automatically generated - may contain errors

Lab products found in correlation

Lsm 880, by Zeiss (2 mentions) Bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Sh30023, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Gapdh antibody, by Boster Bio (1 mentions) Tgf beta 1 antibody, by Proteintech (1 mentions)

4 protocols using fshr antibody

Primary Mouse Granulosa Cells Isolation

Check if the same lab product or an alternative is used in the 5 most similar protocols

For primary mGCs isolation, ovaries were removed from 21 to 23-day-old mice and punctured with 25-gauge needles. The cell suspension was collected and filtered with a 40-µm cell strainer to eliminate oocytes. mGCs were authenticated by immunofluorescence analysis using the FSHR antibody (Proteintech, 22665-1-AP).
The SHP2 knockout KGN cells were engineered by CRISPR/Cas9 technology according to previous reports (Ran et al., 2017 (link)). SHP2-overexpressing adenovirus was added to SHP2-deficient cells and normal cells to restore or enhance the level of SHP2. All cells were cultured with DMEM/F12 medium (HyClone, SH30023.01) with 10% fetal bovine serum (Gibco, 10270), 100 IU/ml penicillin and 100 µg/ml streptomycin (HyClone, SV30010.01), and 10 mIU FSH (National Hormone and Peptide Program) in a 37°C humidified incubator with 5% CO₂.

Wei X., Zheng L., Tian Y., Wang H., Su Y., Feng G., Wang C, & Lu Z. (2022). Tyrosine phosphatase SHP2 in ovarian granulosa cells balances follicular development by inhibiting PI3K/AKT signaling. Journal of Molecular Cell Biology, 14(7), mjac048.

+ Open protocol

+ Expand

Immunofluorescence Analysis of FSHR Expression in Ovarian Granulosa Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Isolated primary ovarian granulosa cells were plated on cell slides and incubated overnight at 37 °C with 95% air and 5% CO2. The culture medium was removed, and the cells were washed once with precooled PBS. Cells were fixed with 4% paraformaldehyde at room temperature for 15 min. Then, the cells were blocked with 5% BSA/0.3% Triton X-100/PBS for 60 min. Next, FSHR antibody (Proteintech, 22665-1-AP) was added at a 1:50 dilution and incubated overnight at 4 °C. After washing three times with PBS, the cells were incubated with a secondary antibody (anti-rabbit Alexa Fluor 488, A32731, Invitrogen) diluted in antibody dilution buffer (1% BSA/0.3% Triton X-100/PBS) at a ratio of 1:2000 for 60 min at room temperature in the dark. Following three washes with PBS, cell slides were mounted with mounting medium with DAPI - Aqueous, Fluoroshield (Abcam, ab104139) and observed using a confocal microscope (ZEISS, LSM 880).

Hu B., Zheng X, & Zhang W. (2024). Resveratrol-βcd inhibited premature ovarian insufficiency progression by regulating granulosa cell autophagy. Journal of Ovarian Research, 17, 18.

+ Open protocol

+ Expand

Immunofluorescent Localization of FSHR

Check if the same lab product or an alternative is used in the 5 most similar protocols

Granulosa cells were fixed with 4% paraformaldehyde for 30 min, treated with TritonX-100 for 20 min, and then incubated with BSA for 30 min. Next, cells were incubated overnight with FSHR antibody (1:300; Proteintech) at 4 °C. Then, cells were incubated in darkness with fluorescent secondary antibody at 37 °C for 2 h. Subsequently, the cells were visualized and photographed using a fluorescence microscope (APExBIO, Houston, TX, USA).

Li Y., Gao Y., Yao D., Li Z., Wang J., Zhang X., Zhao X, & Zhang Y. (2024). Heme Oxygenase-1 Regulates Zearalenone-Induced Oxidative Stress and Apoptosis in Sheep Follicular Granulosa Cells. International Journal of Molecular Sciences, 25(5), 2578.

+ Open protocol

+ Expand

Ovarian Protein Extraction and Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Protein was extracted from the rats’ ovaries using RIPA (radioimmunoprecipitation assay) lysis buffer and was quantified by using a BCA protein assay kit (Thermo Fisher, SE253117A). Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was performed with 10% separation glue. Then the protein on the gel was transferred to polyvinylidene difluoride (PVDF) membrane under the condition of constant 300 mA. 5% skimmed milk dissolved in Tris-buffered saline with Tween (TBST) was used to block the PVDF membrane after transmembrane. The primary antibody was incubated overnight at 4 °C, and the secondary antibody was incubated for 1 h at room temperature. After that, an ECL kit was used to determine chemiluminescence.
The antibodies were TGF-Beta1 Antibody (Proteintech, 21898-1-AP), FSHR Antibody (Proteintech, 22665-1-AP), Anti-Smad4 (Abcam, ab40759), JNK (Phospho-Thr183/Thr183/Thr221) Rabbit pAb (ZENBIO, 384799), JNK Antibody (Proteintech, 10023-1-AP), Phospho-Smad2 (Ser467) Rabbit pAb (ZENBIO, 310079), Smad2/3 Rabbit pAb (ZENBIO, 382472), Phospho-Smad3 (Ser423/425) Rabbit pAb (ZENBIO, 382919), Smad3 Rabbit pAb (ZENBIO, 382774), TAK1 (Phospho-Ser439) Rabbit pAb(ZENBIO, 385849), TAK1 (3G1) Mouse mAb (ZENBIO, 200993), TRAF6 Rabbit pAb (ZENBIO, 385965), p38 MAPK (CST, 8690S), Phospho-p38 MAPK (CST, 4631S), GAPDH Antibody (Boster, BA2913), HRP-linked Antibody (CST, 7074).

Li M., Xie L., Li Y., Liu J., Nie G, & Yang H. (2020). Synergistic effect of Huyang Yangkun Formula and embryonic stem cells on 4-vinylcyclohexene diepoxide induced premature ovarian insufficiency in mice. Chinese Medicine, 15, 83.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!