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Nextseq single read sequencing

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The NextSeq Single-Read sequencing system is a benchtop sequencing instrument designed for high-throughput, single-read DNA sequencing. It utilizes Illumina's sequencing-by-synthesis technology to generate sequencing data. The core function of the NextSeq system is to perform DNA sequencing with high accuracy and efficiency.

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Lab products found in correlation

Easy dna kit, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

NextSeq (1155 citations) NextSeq sequencing (18 citations) NextSeq reads (2 citations)

2 protocols using nextseq single read sequencing

1

Whole Genome Sequencing of Isolates

Check if the same lab product or an alternative is used in the 5 most similar protocols
The frozen inoculum and isolate samples were thawed on ice, genomic DNA was extracted (Easy DNA Kit, Invitrogen) from each sample, followed by processing for Illumina NextSeq Single-Read sequencing. All genome analysis was preformed using CLC Genomics Workbench. Reads were aligned to the CG8421 reference genome for each isolate population. Only isolate population samples with at least 50x coverage of the CG8421 genome were included for genomic variant analysis. These samples averaged >1000x genome coverage (Sup. Fig. 2).
Crofts A.A., Poly F.M., Ewing C.P., Kuroiwa J.M., Rimmer J.E., Harro C., Sack D., Talaat K.R., Porter C.K., Gutierrez R.L., DeNearing B., Brubaker J., Laird R.M., Maue A.C., Jaep K., Alcala A., Tribble D.R., Riddle M.S., Ramakrishnan A., McCoy A.J., Davies B.W., Guerry P, & Trent M.S. (2018). Campylobacter jejuni transcriptional and genetic adaptation during human infection. Nature microbiology, 3(4), 494-502.
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2

Whole Genome Sequencing of Isolates

Check if the same lab product or an alternative is used in the 5 most similar protocols
The frozen inoculum and isolate samples were thawed on ice, genomic DNA was extracted (Easy DNA Kit, Invitrogen) from each sample, followed by processing for Illumina NextSeq Single-Read sequencing. All genome analysis was preformed using CLC Genomics Workbench. Reads were aligned to the CG8421 reference genome for each isolate population. Only isolate population samples with at least 50x coverage of the CG8421 genome were included for genomic variant analysis. These samples averaged >1000x genome coverage (Sup. Fig. 2).
Crofts A.A., Poly F.M., Ewing C.P., Kuroiwa J.M., Rimmer J.E., Harro C., Sack D., Talaat K.R., Porter C.K., Gutierrez R.L., DeNearing B., Brubaker J., Laird R.M., Maue A.C., Jaep K., Alcala A., Tribble D.R., Riddle M.S., Ramakrishnan A., McCoy A.J., Davies B.W., Guerry P, & Trent M.S. (2018). Campylobacter jejuni transcriptional and genetic adaptation during human infection. Nature microbiology, 3(4), 494-502.
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