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Apc conjugated cd107a antibody

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The APC-conjugated CD107a antibody is a laboratory reagent used for the detection and analysis of CD107a, a protein expressed on the surface of cells. The antibody is conjugated with the fluorescent dye allophycocyanin (APC), which allows for the visualization and quantification of CD107a-positive cells using flow cytometry or other fluorescence-based techniques.

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Lab products found in correlation

Ab serum, by Valley Biomedical (1 mentions) U bottom 96 well plate, by Thermo Fisher Scientific (1 mentions) Golgistop containing monensin, by BD (1 mentions) Aim 5, by Thermo Fisher Scientific (1 mentions) Automacs running buffer, by Miltenyi Biotec (1 mentions) Accuri c6 flow cytometer, by BD (1 mentions) Pe conjugated anti cd56 antibodies, by BD (1 mentions) Monensin, by Merck Group (1 mentions) Brefeldin a, by Merck Group (1 mentions) Ionomycin, by Merck Group (1 mentions) Phorbol myristate acetate (pma), by Merck Group (1 mentions)

Close spelling variants of this product

CD107a-APC antibody CD107a-APC

3 protocols using apc conjugated cd107a antibody

1

NK Cell Degranulation Assay

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NK cells were first washed with 1x PBS (Hyclone) once and re-suspended into 1 mL AIM-V (Invitrogen) supplemented with 5% AB serum (Valley Biomedical). APC-conjugated CD107a antibody (BD Biosciences) and Golgi stop containing monensin (BD Biosciences) were added in and mixed up well with the cells. Target cells were washed and re-suspended into AIM-V (Invitrogen) supplemented with 5% AB serum (Valley Biomedical), and subsequently seeded into a U-bottom 96-well plate (Thermo Fisher Scientific) at the concentration of 2 × 105 cells/100 μL/well. NK cells were then co-cultured at a 1:1 E: T ratio with target cells in a 37°C, 5% CO2 incubator for 5 h. After incubation, the cells were used for anti-CD56 PE antibody staining (Miltenyi). The cells were then suspended in 150 μL autoMACS Running Buffer (Miltenyi) for the detection of the CD107a surface expression on NK cells by BD Accuri C6 Flow Cytometer (BD Biosciences).
Du Z., Ng Y.Y., Zha S, & Wang S. (2021). piggyBac system to co-express NKG2D CAR and IL-15 to augment the in vivo persistence and anti-AML activity of human peripheral blood NK cells. Molecular Therapy. Methods & Clinical Development, 23, 582-596.
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2

NK Cell-Mediated Breast Cancer Cytotoxicity

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Purified NK cells (2.5×105) were cultured alone or with breast cancer cell lines in 96-well plates in a 200μl volume. Cells were stimulated with tumor cells and Trastuzumab, with the presence of different mAbs for PVR-like receptors. APC-conjugated CD107a antibody (BD Pharmingen) and Monensin were directly added to the culture medium and co-cultured with cells for 16 h at 37 °C with 5% CO2. After culture, cell mixture was blocked with human serum 100μl for 15 min at 4 °C, and stained with PE-conjugated anti-CD56 antibodies (BD Pharmingen) for 30 min at 4 °C. The cells were washed with the staining buffer and analyzed with flow cytometry.
Xu F., Sunderland A., Zhou Y., Schulick R.D., Edil B.H, & Zhu Y. (2017). Blockade of CD112R and TIGIT signaling sensitizes human natural killer cell functions. Cancer immunology, immunotherapy : CII, 66(10), 1367-1375.
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3

NK and CD8+ T Cell Cytotoxicity Assay

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Freshly isolated NK and CD8+ T cell subsets were incubated at 37°C for 6 h with K562 or C1R-MICA/C1R cells, at a fixed effector to target (E:T) ratio of 2:1, in the presence of APC-conjugated CD107a antibody (BD Biosciences), as previously described68. Brefeldin A (1μg/ml; Sigma-Aldrich) and Monensin (1 μg/ml; Sigma-Aldrich) were added in the final 5h-incubation period. Effector cells incubated alone in the presence phorbol-12-myristate-13-acetate (PMA, 50 ng/ml, Sigma-Aldrich) with ionomycin, (250 ng/ml, Sigma-Aldrich) were used as positive control whereas medium alone served as unstimulated (US) control. After incubation, cells were stained for surface markers for 30 min on ice, followed by intracellular detection of cytokines (TNF-α and IFN-γ) and CD107a expression and analysed by flow cytometry.
Pereira B.I., De Maeyer R.P., Covre L.P., Nehar-Belaid D., Lanna A., Ward S., Marches R., Chambers E.S., Gomes D.C., Riddell N.E., Maini M.K., Teixeira V.H., Janes S.M., Gilroy D.W., Larbi A., Mabbott N.A., Ucar D., Kuchel G.A., Henson S.M., Strid J., Lee J.H., Banchereau J, & Akbar A.N. (2020). Sestrins induce natural killer function in senescent-like CD8+ T cells. Nature immunology, 21(6), 684-694.
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