P65 sc 372x

Manufactured by Santa Cruz Biotechnology

Sourced in United States

The P65 (sc-372X) is a laboratory equipment product offered by Santa Cruz Biotechnology. It serves as a core component in various research applications. The product's primary function is to facilitate specific experimental procedures, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Pu 1 sc 352x, by Santa Cruz Biotechnology (1 mentions) H3k4me1 ab8895, by Abcam (1 mentions) H3k4me3, by Merck Group (1 mentions) α tubulin, by Merck Group (1 mentions) Lamin a c, by Santa Cruz Biotechnology (1 mentions) Ne per nuclear and cytoplasmic extraction kit, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

(sc-372X,

2 protocols using p65 sc 372x

Western Blot and ChIP Antibody Panel

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The following antibodies were used in western blot and ChIP: TRIM33 (3E9, Euromedex for WB (1:1,000); A301-059A, Bethyl for ChIP (1:100)), c-JUN (sc-1694X, Santa Cruz (1:200)), p65 (sc-372X, Santa Cruz (1:200)), RNA Pol II (sc-899X, Santa Cruz (1:200)), H3K4me3 (07-473, Millipore (1:500)), PU.1 (sc-352X, Santa Cruz (1:10,000 for western blot; 1:500 for ChIP)), p300 (sc-585X, Santa Cruz), AcH3 (06-599, Millipore (1:500)), H3K4me1 (ab-8895, Abcam (1:500)), Flag (M2 monoclonal, Sigma Aldrich (1:1,000)), CBP (A-22, sc-369, Santa Cruz (1:1,000)), β-actin (A5441, Sigma Aldrich (1:10,000)).

Ferri F., Parcelier A., Petit V., Gallouet A.S., Lewandowski D., Dalloz M., van den Heuvel A., Kolovos P., Soler E., Squadrito M.L., De Palma M., Davidson I., Rousselet G, & Romeo P.H. (2015). TRIM33 switches off Ifnb1 gene transcription during the late phase of macrophage activation. Nature Communications, 6, 8900.

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Protein Expression Analysis in Cell Fractions

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After the indicated treatments, attached and detached cells were harvested and protein lysates were prepared as previously described [24 (link)]. 20 μg of protein lysates were probed with the following antibodies: p65 (sc-372X, 1:1000, Dallas, TX, Santa Cruz), Lamin A/C (sc-20681, 1:500, Santa Cruz, Dallas, TX, USA), α-tubulin (#T9026, 1:5000, Sigma-Aldrich, St. Louis, MO, USA), Adenovirus 2/5 E1A (sc-430, 1:500, Dallas, TX, Santa Cruz, USA). Cellular fractionation was performed using NE-PER™ Nuclear and Cytoplasmic Extraction Kit (Thermo Fisher Scientific, Waltham, MA, USA) following manufacturer's instruction.

Passaro C., Borriello F., Vastolo V., Di Somma S., Scamardella E., Gigantino V., Franco R., Marone G, & Portella G. (2015). The oncolytic virus dl922-947 reduces IL-8/CXCL8 and MCP-1/CCL2 expression and impairs angiogenesis and macrophage infiltration in anaplastic thyroid carcinoma. Oncotarget, 7(2), 1500-1515.

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