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Fluoview version 1.7c software

Manufactured by Olympus
Sourced in Japan

Fluoview Version 1.7c software is a microscope imaging software developed by Olympus. It is designed to control and acquire data from Olympus fluorescence microscopes. The software provides basic functions for image capture, processing, and analysis.

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2 protocols using fluoview version 1.7c software

1

Double Immunofluorescence Staining of B-Cells

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Double immunofluorescence staining was performed as previously described.36 (link) Briefly, B-cells were fixed in 4% paraformaldehyde (15 min, RT), washed with PBS, permeabilized (0.1% Triton X-100 in PBS, 15 min), and attached on pre-coated poly-L-lysine slides. Slides were blocked in 3% BSA, followed by mouse monoclonal anti-NS3 (Abcam; 1:100, ab65407) or anti-core (ThermoFisher Scientific, Waltham, MA, USA; 1:100, MA1-080) antibody staining. The cells were further incubated with rabbit polyclonal anti-CD19, anti-CD20 (Cell Signaling Technology, Inc., Danvers, MA, USA; 1:400; 3574), anti-CD20 (Abcam; 1:400; ab78237) or anti-B220 antibody (Biolegend, San Diego, CA, USA; 1:200; #103201). Cells were washed with PBS and incubated with goat anti-rabbit Alexa Fluor 488 and goat anti-mouse or anti-rat Alexa Fluor 597 (Invitrogen; 1:200). The stained slides were mounted using Vectashield mounting medium (Vector Laboratories Inc., Burlingame, CA, USA). Control slides were similarly processed, except primary antisera were omitted, which yielded no staining. Images were captured using an Olympus FV1000 confocal microscope and processed with Olympus Fluoview Version 1.7c software (Olympus Corporation, Tokyo, Japan).
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2

Immunostaining of Hepatitis C Virus Proteins

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Double immunofluorescence staining was performed as previously described.36 (link) Briefly, B cells were fixed in 4% paraformaldehyde (15 min, room temperature), washed with phosphate-buffered saline, permeabilized (0.1% Triton X-100 in phosphate-buffered saline, 15 min) and attached on precoated poly-L-lysine slides. Slides were blocked in 3% bovine serum albumin, followed by mouse monoclonal anti-NS3 (Abcam; 1:100, ab65407) or anti-core (ThermoFisher Scientific; 1:100, MA1-080) antibody staining. The cells were further incubated with rabbit polyclonal anti-CD19, anti-CD20 (Cell Signaling Technology, Inc., Danvers, MA, USA; 1:400; 3574), anti-CD20 (Abcam; 1:400; ab78237) or anti-B220 antibody (Biolegend; 1:200; #103201). Cells were washed with phosphate-buffered saline and incubated with goat anti-rabbit Alexa Fluor 488 and goat anti-mouse or anti-rat Alexa Fluor 597 (Invitrogen; 1:200). The stained slides were mounted using Vectashield mounting medium (Vector Laboratories Inc., Burlingame, CA, USA). Control slides were similarly processed, except primary antisera were omitted, which yielded no staining. Images were captured using an Olympus FV1000 confocal microscope and processed with Olympus Fluoview Version 1.7c software (Olympus Corporation, Tokyo, Japan).
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