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2 protocols using 6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid trolox hydrochloric acid

1

Biochemical Analysis of Antioxidant Compounds

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Analytical-grade methanol, acetonitrile, and water were purchased from Fisher Scientific (Pittsburgh, PA, USA). The reagent-grade chemicals including 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (trolox), hydrochloric acid, potassium persulfate, 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), hydrochloride, 2,4,6-tris(2-pyridyl)-trizine (TPTZ), iron(III) chloride hexahydrate, sodium acetate, acetic acid, sodium carbonate, sodium hydroxide, formic acid, methoxyamine hydrochloride, pyridine, and N-methyl-N-(trimethylsilyl)-trifluoroacetamide (MSTFA) were obtained from Sigma Chemical Co. (St. Louis, MO, USA).
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2

Measurement of Antioxidant Capacity

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2,4,6-Tris(2-pyridyl)-1,3,5-triazine (TPTZ), 2,2-Diphenyl-1-Picrylhydrazyl (DPPH), 6-hydroxy 2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), hydrochloric acid (HCl, 37%), methanol (99,8%), acetone, sodium nitrite, sodium hydroxide Folin–Ciocalteau reagent, maltodextrin and standards were purchased from Sigma-Aldrich (St. Louis, MO, USA). Gallic acid and sodium carbonate from taken from Riedel-de Haen. Sodium acetate and glacial acetic acid were taken from Carlo Erba. Aluminum chloride and iron chloride were purchased from Merck.
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