Anti acetylated lysine

Etoposide, MMS, TSA, NIM, and thymidine were purchased from Sigma-Aldrich. Hydrogen peroxide (H₂O₂) was purchased from Duksan Pure Chemicals (Ansan, Korea). The following antibodies were used in this study: anti-XRCC5 (sc-5280; Santa Cruz Biotechnology, Santa Cruz, CA, USA; 1:1000), anti-XRCC6 (sc-55505; Santa Cruz Biotechnology; 1:1000), anti-β-actin (sc-47778; Santa Cruz Biotechnology; 1:3000), anti-γ-H2AX (#9718S; Cell Signaling Technology, Danvers, MA, USA; 1:1000), anti-PARP-1 (sc-74469; Santa Cruz Biotechnology; 1:1000), anti-α-tubulin (LF-PA0146; AbFrontier, Seoul, Korea; 1:1000), anti-SIRT1 (sc-74504; Santa Cruz Biotechnology; 1:1000), anti-Myc (#2276 S; Cell Signaling Technology; 1:1000), anti-Flag (#2368S; Cell Signaling Technology; 1:1000), anti-HA (sc-805; Santa Cruz Biotechnology; 1:1000), anti-His (sc-803; Santa Cruz Biotechnology; 1:1000), anti-acetylated lysine (sc-32268; Santa Cruz Biotechnology; 1:1000), and anti-phosphorylated serine (sc-81514; Santa Cruz Biotechnology; 1:1000). Polyclonal anti-FOXL2 antibodies (1:1000) were generated using either recombinant human FOXL2 protein or N-terminus peptides as an antigen^{51 (link)}. Other reagents were purchased from Sigma-Aldrich, unless otherwise indicated. Antibodies were validated for the specific species and application in pilot studies.

The proteins were isolated from the frozen kidneys and the concentration was calculated by the Bradford method. Immunoblotting examination was performed as previously described [21 ]. In this study, the primary antibodies used were anti-Ecadherin (1:1000,Abcam,UK), anti-ColIII (11,000, Abcam, UK),anti-caspase1. (1:1000, Abcam,UK), anti-TGFβR1 (1:1000,Abcam,UK), anti-Sirt1 (11,000, Abcam, UK), anti-NLRP3 (1:1000, Abclonal, China), anti-IL-1β (11,000,Abclonal,China), anti-Acetylated-lysine (1:500,Santa Cruz,USA), anti-ASC (1:500, Santa Cruz, USA), anti-TGF-β1 (1500,Santa Cruz,USA), anti-Smad3 (1,1000,CST,USA), and anti-Gapdh (12,000, Proteintech, USA). Protein bands were visualized using the chemiluminescence system (Tanon) for the required time. Quantitative analysis was performed using ImageJ software.