Cells were enzymatically digested using serial digestions of collagenase type 1 and 2 (Worthington), and plated onto 8 well chamber slides (Lab-Tek, Sigma-Aldrich). The cells were allowed to attach for 72hrs and imaged using phase contrast microscopy, Cell area was quantified from these images using the “%Area” function in Image J software after thresholding of the cells in each image. Cell elongation ratio was calculated from these images using the “Roundness” function in Image J where the aspect ratio was defined as (1–Roundness), with 0 corresponding to a circle and 1 corresponding to a completely elongated object30 (link).
Collagenase type 1 and 2
Manufactured by Worthington
Collagenase type 1 and 2 are enzymes used for the digestion and dissociation of collagen-rich tissues. Type 1 collagenase primarily targets the triple-helical region of collagen, while type 2 collagenase is more specific for the telopeptide regions. These enzymes are commonly used in cell isolation and tissue dissociation protocols.
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Lab products found in correlation
2 protocols using collagenase type 1 and 2
1
Quantifying Cell Morphology from Images
2
Quantifying Cell Morphology from Images
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Cells were enzymatically digested using serial digestions of collagenase type 1 and 2 (Worthington), and plated onto 8 well chamber slides (Lab-Tek, Sigma-Aldrich). The cells were allowed to attach for 72hrs and imaged using phase contrast microscopy, Cell area was quantified from these images using the “%Area” function in Image J software after thresholding of the cells in each image. Cell elongation ratio was calculated from these images using the “Roundness” function in Image J where the aspect ratio was defined as (1–Roundness), with 0 corresponding to a circle and 1 corresponding to a completely elongated object30 (link).
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