The human chondrosarcoma cell line SW 1353 was acquired from the American Type Culture Collection (HTB-94, ATCC Manassas, VA, USA) and cultured in Dulbecco's Modified Eagle Medium (12800-058 GIBCO, Auckland NZ) supplemented with 10 % fetal bovine serum (FBS) (S1560-500 Biowest, Riverside, MO, USA) and 1 % penicillin-streptomycin (15240-062 GIBCO, NY, USA) in a humidified incubator at 37 °C and 5 % CO2. Once SW 1353 cells reached a confluency of about 80 %, they were trypsinized and cultured in flasks according to the experiment: 1 × 104 cells in 96-well culture plates for proliferation assays; 2.1 × 106 cells in T75 flasks for Western Blot (WB); 2.5 × 105 cells in 6-well culture plates for semi-quantitative RT-PCR. All experiments were performed at least in triplicate, culturing SW 1353 cells in three different conditions: in supplemented medium (supplied), without FBS (starvation), or without FBS adding L-β-hydroxybutyrate (β-HB) (Sigma-Aldrich, Toluca, Mexico) at concentrations of 10 or 25 mM, for 12, 24, or 48 h.
Sw1353
Manufactured by Merck Group
Sourced in United States
The SW1353 is a laboratory equipment product manufactured by Merck Group. It serves as a core function for various research and analytical applications. The detailed specifications and intended use of this product are not available at this time.
Automatically generated - may contain errors
Lab products found in correlation
Sw1353, by American Type Culture Collection (6 mentions)
Sw1353, by Thermo Fisher Scientific (3 mentions)
Pbabe retroviral vector, by Cell Biolabs (3 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (3 mentions)
Polybrene, by Merck Group (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Azacytidine, by Cayman Chemical (1 mentions)
Fk866, by Merck Group (1 mentions)
Virapower hiperform t rex gateway expression system, by Thermo Fisher Scientific (1 mentions)
Ht1080, by Merck Group (1 mentions)
Hct 116, by ScienCell (1 mentions)
Decitabine, by Cayman Chemical (1 mentions)
Mcf 10a, by ScienCell (1 mentions)
Gmx1778, by Cayman Chemical (1 mentions)
Pge2 elisa kit, by Cayman Chemical (1 mentions)
Tacs xtt cell proliferation kit, by R&D Systems (1 mentions)
Thp 1, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Tnf α, by R&D Systems (1 mentions)
8 protocols using sw1353
1
Chondrosarcoma Cell Line Culture
2
Engineered Glioblastoma TICs and Xenografts
3
Chondrosarcoma and Monocyte Cell Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SW1353 human chondrosarcoma and THP-1 human monocytic cell lines were procured from American Type Culture Collection (ATCC, Manassas, VA). SW1353 and THP-1 cells were grown in Dulbecco's modified Eagle's medium (DMEM) (Sigma-Aldrich, St. Louis, MO) containing 10% fetal bovine serum (Thermo-Fisher Scientific, Waltham, MA). PromoCell (Heidelberg, Germany) was the source of human primary chondrocyte (HCH) cells and the chondrocyte growth medium. The experiments in the present study utilized the cultured chondrocytes of passage numbers between five and eight. The cells were passaged at about 80% confluence.
TNF-α, LTB4 ELISA kits, and TACS XTT Cell Proliferation kit were procured from R&D Systems (Minneapolis, MN). PGE2 ELISA kit was purchased from Cayman Chemical (Ann Arbor, MI). Corning “V” bottom culture plates, dimethyl sulfoxide (DMSO), 1,9-Dimethylmethylene Blue zinc chloride double salt (DMMB), Lipopolysaccharides (LPS) from Escherichia coli O55: B5 (LPS), A23187, and remaining analytical grade reagents were purchased from Sigma-Aldrich (St. Louis, MO). Alexafluor® 647 tagged mouse anti-human SOX-9 antibody; True Nuclear Transcription factor buffer set was procured from BD PharmingenTM (Franklin Lakes, NJ).
TNF-α, LTB4 ELISA kits, and TACS XTT Cell Proliferation kit were procured from R&D Systems (Minneapolis, MN). PGE2 ELISA kit was purchased from Cayman Chemical (Ann Arbor, MI). Corning “V” bottom culture plates, dimethyl sulfoxide (DMSO), 1,9-Dimethylmethylene Blue zinc chloride double salt (DMMB), Lipopolysaccharides (LPS) from Escherichia coli O55: B5 (LPS), A23187, and remaining analytical grade reagents were purchased from Sigma-Aldrich (St. Louis, MO). Alexafluor® 647 tagged mouse anti-human SOX-9 antibody; True Nuclear Transcription factor buffer set was procured from BD PharmingenTM (Franklin Lakes, NJ).
4
Skin and Arthritis Cell Models
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Normal dermal fibroblast cell line (BJ, CRL-2522) and chondrosarcoma cell line (SW1353, HTB-94) were purchased from American Type Culture Collection. Dermal fibroblasts have been previously used in investigations of borrelial infection (Georgilis et al., 1992 (link)). Furthermore, the use of chondrosarcoma cells in osteoarthritis research is well documented (Chen et al., 2017 (link); Liu et al., 2017 (link)). Therefore, these cell lines, BJ and SW1353, were utilized for their relevance as a disease-related model for skin manifestations and arthritis, respectively. The SW1353 cells were grown at +37°C with 100% air in Leibovitz’s L-15 media (Sigma), while the BJ cells were at +37°C, 5% CO2 in Eagle’s minimum essential media (Sigma) as instructed by the manufacturers. Both media were supplemented with 10% fetal bovine serum (Gibco), 2 mM L-glutamine (Gibco), and 100 IU/ml penicillin/0.2 mg/ml streptomycin (Gibco) antibiotic cocktail. Sodium pyruvate (1 mM, Gibco) was also added to the BJ media.
5
Culturing Human Chondrocytes and Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human CS cell line (SW1353) was purchased from the American Type Culture Collection. Primary HACs were separated from normal cartilage of non-osteoarthritic joints at Maharaj Nakorn Chiang Mai Hospital with informed consent and local ethical committee approval (Ethic approval no. ORT-11-09-16A-14). SW1353 and HAC cells were cultured in DMEM (Sigma) supplemented with 10% fetal bovine serum (FBS) (Sigma), penicillin (100 U/mL) (Sigma), streptomycin (100 μg/mL) (Sigma), and L-glutamine (2 mM) (Sigma), and maintained in a humidified atmosphere of 37°C in a 5% CO2. HAC cells were used for up to four passages.
6
Modulating HOTAIR expression in chondrosarcoma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human chondrosarcoma cells (SW1353) were obtained from the American Type Culture Collection (ATCC). Cells were grown in maintenance medium consisting of DMEM supplemented with 10% FBS at 37°C with 5% CO 2 .
To silence HOTAIR function, SW1353 cells were transfected with small interfering RNA (siRNA) oligonucleotides targeting HOTAIR or the negative control (50 nM), using Lipofectamine™ RNAiMAX (Invitrogen, USA) according to the manufacturer's instructions. The gene-specific siRNA is siHOTAIR (5'-GAACGGGAGUACAGAGAGAUU-3'). Transfected cells were kept in maintenance medium for 48 hours prior to further analyses.
To overexpress HOTAIR in SW1353 cells, a HOTAIR expression retrovirus vector was constructed. Full-length HOTAIR was amplified by PCR and cloned into the pBABE retroviral vector (Cell Biolabs, USA) using the primers 5'-GACTCGCCTGTGCTCTGGAGCT-3' and 5'-TTGAAAATGCATCCAGATTTTT-3'. SW1353 cells were infected with retrovirus containing HOTAIR or negative control (vector) in the presence of 10 μg/mL polybrene (Sigma-Aldrich, USA). The supernatant was removed after 24 hours and replaced with maintenance medium containing 1μg/mL puromycin. Cells were cultured for 48 hours prior to further analyses.
To silence HOTAIR function, SW1353 cells were transfected with small interfering RNA (siRNA) oligonucleotides targeting HOTAIR or the negative control (50 nM), using Lipofectamine™ RNAiMAX (Invitrogen, USA) according to the manufacturer's instructions. The gene-specific siRNA is siHOTAIR (5'-GAACGGGAGUACAGAGAGAUU-3'). Transfected cells were kept in maintenance medium for 48 hours prior to further analyses.
To overexpress HOTAIR in SW1353 cells, a HOTAIR expression retrovirus vector was constructed. Full-length HOTAIR was amplified by PCR and cloned into the pBABE retroviral vector (Cell Biolabs, USA) using the primers 5'-GACTCGCCTGTGCTCTGGAGCT-3' and 5'-TTGAAAATGCATCCAGATTTTT-3'. SW1353 cells were infected with retrovirus containing HOTAIR or negative control (vector) in the presence of 10 μg/mL polybrene (Sigma-Aldrich, USA). The supernatant was removed after 24 hours and replaced with maintenance medium containing 1μg/mL puromycin. Cells were cultured for 48 hours prior to further analyses.
7
Modulation of HOTAIR in Chondrosarcoma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human chondrosarcoma cells (SW1353) were obtained from the American Type Culture Collection (ATCC). Cells were grown in maintenance medium consisting of DMEM supplemented with 10% FBS at 37°C with 5% CO 2 .
To silence HOTAIR function, SW1353 cells were transfected with small interfering RNA (siRNA) oligonucleotides targeting HOTAIR or the negative control (50 nM), using Lipofectamine™ RNAiMAX (Invitrogen, USA) according to the manufacturer's instructions. The gene-speci c siRNA is siHOTAIR (5'-GAACGGGAGUACAGAGAGAUU-3'). Transfected cells were kept in maintenance medium for 48 hours prior to further analyses.
To overexpress HOTAIR in SW1353 cells, a HOTAIR expression retrovirus vector was constructed. Fulllength HOTAIR was ampli ed by PCR and cloned into the pBABE retroviral vector (Cell Biolabs, USA) using the primers 5'-GACTCGCCTGTGCTCTGGAGCT-3' and 5'-TTGAAAATGCATCCAGATTTTT-3'. SW1353 cells were infected with retrovirus containing HOTAIR or negative control (vector) in the presence of 10 μg/mL polybrene (Sigma-Aldrich, USA). The supernatant was removed after 24 hours and replaced with maintenance medium containing 1μg/mL puromycin. Cells were cultured for 48 hours prior to further analyses.
To silence HOTAIR function, SW1353 cells were transfected with small interfering RNA (siRNA) oligonucleotides targeting HOTAIR or the negative control (50 nM), using Lipofectamine™ RNAiMAX (Invitrogen, USA) according to the manufacturer's instructions. The gene-speci c siRNA is siHOTAIR (5'-GAACGGGAGUACAGAGAGAUU-3'). Transfected cells were kept in maintenance medium for 48 hours prior to further analyses.
To overexpress HOTAIR in SW1353 cells, a HOTAIR expression retrovirus vector was constructed. Fulllength HOTAIR was ampli ed by PCR and cloned into the pBABE retroviral vector (Cell Biolabs, USA) using the primers 5'-GACTCGCCTGTGCTCTGGAGCT-3' and 5'-TTGAAAATGCATCCAGATTTTT-3'. SW1353 cells were infected with retrovirus containing HOTAIR or negative control (vector) in the presence of 10 μg/mL polybrene (Sigma-Aldrich, USA). The supernatant was removed after 24 hours and replaced with maintenance medium containing 1μg/mL puromycin. Cells were cultured for 48 hours prior to further analyses.
8
Modulation of HOTAIR Expression in Human Chondrosarcoma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human chondrosarcoma cells (SW1353) were obtained from the American Type Culture Collection (ATTC). Cells were grown in maintenance medium consisting of DMEM supplemented with 10% FBS at 37 °C with 5% CO 2 .
To silence HOTAIR function, SW1353 cells were transfected with small interfering RNA (siRNA) oligonucleotides targeting HOTAIR or the negative control (50 nM), using Lipofectamine™ RNAiMAX (Invitrogen, USA) according to the manufacturer's instructions. The gene-speci c siRNA is siHOTAIR (5'-GAACGGGAGUACAGAGAGAUU-3'). Transfected cells were kept in maintenance medium for 48 hours prior to further analyses.
To overexpress HOTAIR in SW1353 cells, a HOTAIR expression retrovirus vector was constructed. Fulllength HOTAIR was ampli ed by PCR and cloned into the pBABE retroviral vector (Cell Biolabs, USA) using the primers 5'-GACTCGCCTGTGCTCTGGAGCT-3' and 5'-TTGAAAATGCATCCAGATTTTT-3'. SW1353 cells were infected with retrovirus containing HOTAIR or negative control (vector) in the presence of 10 µg/mL polybrene (Sigma-Aldrich, USA). The supernatant was removed after 24 hours and replaced with maintenance medium containing 1 µg/mL puromycin. Cells were cultured for 48 hours prior to further analyses.
To silence HOTAIR function, SW1353 cells were transfected with small interfering RNA (siRNA) oligonucleotides targeting HOTAIR or the negative control (50 nM), using Lipofectamine™ RNAiMAX (Invitrogen, USA) according to the manufacturer's instructions. The gene-speci c siRNA is siHOTAIR (5'-GAACGGGAGUACAGAGAGAUU-3'). Transfected cells were kept in maintenance medium for 48 hours prior to further analyses.
To overexpress HOTAIR in SW1353 cells, a HOTAIR expression retrovirus vector was constructed. Fulllength HOTAIR was ampli ed by PCR and cloned into the pBABE retroviral vector (Cell Biolabs, USA) using the primers 5'-GACTCGCCTGTGCTCTGGAGCT-3' and 5'-TTGAAAATGCATCCAGATTTTT-3'. SW1353 cells were infected with retrovirus containing HOTAIR or negative control (vector) in the presence of 10 µg/mL polybrene (Sigma-Aldrich, USA). The supernatant was removed after 24 hours and replaced with maintenance medium containing 1 µg/mL puromycin. Cells were cultured for 48 hours prior to further analyses.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!