HPLC-grade Acetonitrile and water were purchased by Panreac (Madrid, Spain). Standards of kaempferol, myricetin, quercetin, rutin, caffeic, chlorogenic, and protocautechic acids of HPLC-grade were purchased by Sigma-Aldrich (Madrid, Spain). We also acquired CCCP from Sigma (C2759).
Tert-butylhydroperoxide (t-BOOH), glutathione reductase (GR), reduced (GSH) and oxidized (GSSG) glutathione, dichlorofluorescin (DCFH), o-phthaldialdehyde (OPT), nicotine adenine dinucleotide phosphate reduced salt (NADPH), 2,4-dinitrophenylhydrazine (DNPH), H2O2, 1,1,3,3-tetraethoxypropane (TEP), gentamicin, penicillin G, and streptomycin were purchased from Sigma Chemical Co. (Madrid, Spain), and rapamycin was purchased from Merck (number 553,210). Acetonitrile, methanol of HPLC grade, dimethyl sulfoxide (DMSO) of analytical grade, sodium hydroxide, sodium chloride, di-sodium hydrogen phosphate anhydrous, potassium di-hydrogen phosphate, as well as formic, hydrochloric, perchloric, and sulfuric acids were acquired from Panreac (Barcelona, Spain). Bradford reagent was from BioRad Laboratories S.A. DMEM culture media and fetal bovine serum (FBS) were from Cultek (Madrid, Spain). All other reagents were of analytical quality.
Tert-butylhydroperoxide (t-BOOH), glutathione reductase (GR), reduced (GSH) and oxidized (GSSG) glutathione, dichlorofluorescin (DCFH), o-phthaldialdehyde (OPT), nicotine adenine dinucleotide phosphate reduced salt (NADPH), 2,4-dinitrophenylhydrazine (DNPH), H2O2, 1,1,3,3-tetraethoxypropane (TEP), gentamicin, penicillin G, and streptomycin were purchased from Sigma Chemical Co. (Madrid, Spain), and rapamycin was purchased from Merck (number 553,210). Acetonitrile, methanol of HPLC grade, dimethyl sulfoxide (DMSO) of analytical grade, sodium hydroxide, sodium chloride, di-sodium hydrogen phosphate anhydrous, potassium di-hydrogen phosphate, as well as formic, hydrochloric, perchloric, and sulfuric acids were acquired from Panreac (Barcelona, Spain). Bradford reagent was from BioRad Laboratories S.A. DMEM culture media and fetal bovine serum (FBS) were from Cultek (Madrid, Spain). All other reagents were of analytical quality.