Pre-wetted TIPS microspheres were mounted on sterile glass slides using super glue and imaged under deionized water in intermittent contact (liquid) mode employing a JPK NanoWizard II AFM (JPK Instruments, Germany) mounted on an Olympus IX71 inverted microscope. MSNL-10 cantilevers (Bruker, Santa Barbara, USA), tuned to a drive frequency of ∼46 kHz (nominal resonant frequency 25–50 kHz), were employed with a constant line rate of 0.5 Hz. Gain parameters and set point were adjusted according to each sample. Thermal resonance calibration yielded a cantilever spring constant of 0.11 N/m (nominal value 0.1 N/m). After focusing on an area of interest, images with different scan sizes of 5 × 5 µm were obtained at random sites of each sample and processed with the Gwyddion 2.37 SPM software.
Jpk nanowizard 2 afm
Manufactured by Bruker
Sourced in Germany
The JPK NanoWizard II AFM is an atomic force microscope that provides high-resolution imaging and nanomechanical characterization of surfaces and samples. It offers advanced functionalities for topography, force spectroscopy, and various imaging modes.
Automatically generated - may contain errors
Lab products found in correlation
6 protocols using jpk nanowizard 2 afm
1
Atomic Force Microscopy of Pre-wetted TIPS Microspheres
2
Atomic Force Microscopy of Red Blood Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Experiments were carried out at room temperature (23 °C) in a JPK Nanowizard II AFM (JPK Instruments, Berlin, Germany). Images were captured at 1 Hz with 512 × 512 pixels. For this purpose, V-shaped MLCT silicon nitride cantilevers were used (Bruker AXS, Karlsruhe, Germany) in contact mode, with enough force to reduce the overestimation of cell diameters at the edges without compromising cell integrity. Images were collected at 512 × 512-pixel resolution at a scanning rate of 1 Hz and line-fitted using the JPK Data Processing software as required. Cell diameters were measured on the images along the Y and X axis for each cell, combining both data (as RBC have almost circular shape when attached to support). Cell thickness was measured by cross-section analysis of the RBC images.
3
Atomic Force Microscopy of Osteogenic Cells
4
Measurement of Scaffold Modulus by AFM
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The
surface Young’s moduli of 3D printed scaffolds of either CNF
or CNF/GelMA scaffolds were measured using a JPK Nanowizard II AFM
(JPK Instruments AG, Berlin, Germany). Force measurements were performed
in PBS using a DNP-S10 silicon nitride cantilever (spring constant
∼0.06 N m–1) with a 10 nm round tip. The
spring constant of each cantilever was calibrated using the thermal
method, and the deflection sensitivity was determined by performing
a force measurement on a clean glass slide in PBS prior to undertaking
measurements on the scaffolds. Force measurements were performed at
a minimum of three different locations on each scaffold, with five
separate measurements taken at each location and averaged. Three separate
scaffolds were analyzed for each material.
surface Young’s moduli of 3D printed scaffolds of either CNF
or CNF/GelMA scaffolds were measured using a JPK Nanowizard II AFM
(JPK Instruments AG, Berlin, Germany). Force measurements were performed
in PBS using a DNP-S10 silicon nitride cantilever (spring constant
∼0.06 N m–1) with a 10 nm round tip. The
spring constant of each cantilever was calibrated using the thermal
method, and the deflection sensitivity was determined by performing
a force measurement on a clean glass slide in PBS prior to undertaking
measurements on the scaffolds. Force measurements were performed at
a minimum of three different locations on each scaffold, with five
separate measurements taken at each location and averaged. Three separate
scaffolds were analyzed for each material.
5
Polymer Film Surface Morphology Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The morphology of the polymer film surfaces were explored using a JPK Nanowizard II AFM (JPK Instruments AG, Germany). Images were obtained in air using intermittent contact mode AFM with a Nanoworld silica nitride cantilever (spring constant 40 N/m). Image scan of 5 µm×5 µm were obtained at a scan rate of 1 Hz. Roughness values and total surface area measurements were calculated using SPM Image Processing software V.3 (JPK Instruments AG, Germany) and Gwyddion SPM software (version 2.34).
6
Measuring Cell/Tissue Stiffness with AFM
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For measurements of cell/tissue stiffness, a JPK NanoWizard II AFM with BioCell (JPK Instruments, Berlin, Germany) was equipped and manipulated as previously described (Chiou et al., 2013; Harn et al., 2015; Lin et al., 2015) and as detailed in the Supplementary Materials and Methods.
Preparation and fabrication of PA gels PA gels with uniform stiffness were prepared as previously described (Chen et al., 2014; Lin et al., 2015) and as detailed in the Supplementary Materials and Methods. The Young's moduli of the PA gels used were as follows: 2-and 20-kPa PA gels represented the elastic modulus of normal and keloid dermis, respectively. PA gels from each polymerization batch were checked to verify consistent matrix mechanical properties by AFM.
Preparation and fabrication of PA gels PA gels with uniform stiffness were prepared as previously described (Chen et al., 2014; Lin et al., 2015) and as detailed in the Supplementary Materials and Methods. The Young's moduli of the PA gels used were as follows: 2-and 20-kPa PA gels represented the elastic modulus of normal and keloid dermis, respectively. PA gels from each polymerization batch were checked to verify consistent matrix mechanical properties by AFM.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!