Donkey anti rabbit igg h l highly cross adsorbed secondary antibody

Manufactured by Thermo Fisher Scientific

The Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody is a laboratory reagent used to detect and visualize rabbit immunoglobulin G (IgG) in various immunological applications. It is a secondary antibody, meaning it binds to the primary rabbit antibody, allowing for the detection of the target antigen.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Lamp2, by Abcam (1 mentions) Torin 1, by Cell Signaling Technology (1 mentions) Ecl developer, by Beyotime (1 mentions) Tgfbi, by Abcam (1 mentions) Lysotracker green dnd 26, by Cell Signaling Technology (1 mentions) Bafilomycin a1, by Merck Group (1 mentions) Fetal bovine serum (fbs), by ExCell Bio (1 mentions) Gapdh, by Proteintech (1 mentions) Hrp conjugated secondary antibody, by ZSGB-BIO (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Alexa fluor 568, by Thermo Fisher Scientific (1 mentions) Trueblack lipofuscin autofluorescence quencher, by Biotium (1 mentions) Ebioscience 1x rbc lysis buffer, by Thermo Fisher Scientific (1 mentions) Lipopolysaccharides lps from escherichia coli o111 b4, by Merck Group (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Bleomycin hydrochloride, by Nippon Kayaku (1 mentions)

Close spelling variants of this product

Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody(Polyclonal) Anti-rabbit IgG (H + L) highly cross-adsorbed secondary antibody Donkey anti-rabbit IgG (H+L) secondary antibody Donkey anti-rabbit IgG (H + L) Donkey anti-rabbit IgG secondary antibody Donkey anti-rabbit secondary antibody H + L IgG Anti-rabbit secondary antibody IgG Rabbit

3 protocols using donkey anti rabbit igg h l highly cross adsorbed secondary antibody

Macrophage Differentiation and Activation

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The following antibodies were obtained from Cell Signaling Technology: PKD1, PKD2, PKD3 (D57E6 Rabbit mAb), p-PKD1 (Ser916), CD45, CD68, and α-Smooth Muscle Actin. p-PKD2 (Ser876) and eBioscience 1X RBC Lysis Buffer were obtained from Thermo Fisher. TrueBlack Lipofuscin Autofluorescence Quencher was obtained from Biotium. Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488, Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568, and Mouse M-CSF Recombinant Protein were obtained from Invitrogen. Lipopolysaccharides (LPS) from Escherichia coli O111:B4 were purchased from Sigma. Bleomycin hydrochloride was purchased from Nippon Kayaku.

Chen L., Zhao J., Chao Y., Roy A., Guo W., Qian J., Xu W., Domsic R.T., Lafyatis R., Lu B., Deng F, & Wang Q.J. (2023). Loss of Protein Kinase D2 Activity Protects Against Bleomycin-Induced Dermal Fibrosis in Mice. Laboratory investigation; a journal of technical methods and pathology, 103(2), 100018.

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Autophagy Regulation by TGFBI and TFEB

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The following reagents and antibodies were used in this investigation: Dulbecco’s Modified Eagle Media: Nutrient Mixture F-12 (Hyclone, SH30023.01); Fetal bovine serum (ExCell Bio, FND500); ubiquitin (Jingjie PTM BioLab, PTM-1107); TGFBI (Abcam, ab170874); SQSTM1/p62 (Becton, Dickinson and Company, 610,833); CTSD (Calbiochem, IM16); LC3 (Cell Signaling Technology, 3868); LAMP2 (Abcam, ab25631); TFEB (Cell Signaling Technology, 4240); TFEB (Abcam, ab270604); GAPDH (Protein Tech, 10,494-1-AP); HRP-conjugated secondary antibody (Zsbio, ZB-2301); Donkey anti-Rabbit IgG (H + L) highly cross-adsorbed secondary antibody (Invitrogen, A32808); Donkey anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody (Invitrogen, A32789); ECL developer (Beyotime, P0018AS); Bafilomycin A₁ (Sigma, B1793); Torin 1 (Cell Signaling Technology, 14,379); LysoTracker Green DND-26 (Cell Signaling Technology, 8783).

Wang L., Zhao C., Zheng T., Zhang Y., Liu H., Wang X., Tang X., Zhao B, & Liu P. (2021). Torin 1 alleviates impairment of TFEB-mediated lysosomal biogenesis and autophagy in TGFBI (p.G623_H626del)-linked Thiel-Behnke corneal dystrophy. Autophagy, 18(4), 765-782.

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Immunofluorescence and Cell Viability Assays

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SMMC-7721, A549, and HCT116 cells were obtained and cultured as previously reported (39 ). RPMI 1640 (HyClone), high-glucose Dulbecco’s modified Eagle’s medium (HyClone), fetal bovine serum (Gibco), 0.25% trypsin (HyClone), and penicillin-streptomycin (Thermo Fisher Scientific) were used for cell cultures and passages. Goat monoclonal anti-CD31 (R&D Systems, catalog no. AF3628), rabbit monoclonal anti-Iba1 (Wako, catalog no. 019-19741), rabbit polyclonal anti-CA9 (Novus Biologicals, catalog no. NB100-417), and rat monoclonal anti-Ki67 (eBioscience, catalog no. 14-5698) were used as primary antibodies. Secondary antibodies used for paraffin section immunofluorescence were purchased from Invitrogen, including donkey anti-goat immunoglobulin G (IgG) (H + L) cross-adsorbed secondary antibody, Alexa Fluor 555; donkey anti-rabbit IgG (H + L) highly cross-adsorbed secondary antibody, Alexa Fluor 488; and goat anti-rat IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 555. In situ cell death detection kits for TUNEL assays were purchased from Roche (catalog no. 11684795910). Cholecystokinin 8 (CCK8) assay kits were from Biotime Biotechnology. Calcein AM/PI (propidium iodide) were obtained from Sigma-Aldrich.

Chen S., Xing C., Huang D., Zhou C., Ding B., Guo Z., Peng Z., Wang D., Zhu X., Liu S., Cai Z., Wu J., Zhao J., Wu Z., Zhang Y., Wei C., Yan Q., Wang H., Fan D., Liu L., Zhang H, & Cao Y. (2020). Eradication of tumor growth by delivering novel photothermal selenium-coated tellurium nanoheterojunctions. Science Advances, 6(15), eaay6825.

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