Tead1 sirna

Antibodies from Cell Signaling Technology used in this study were against TAZ (#83669; dilution 1:1000), Runx2 (#12556; dilution 1:1000), and GAPDH (#5174; dilution 1:1000). BrdU (#sc-32323; dilution 1:1000), flag (#sc-7945; dilution 1:1000), and GFP (#sc-9996; dilution 1:1000) antibodies were obtained from Santa Cruz Biotechnology. Sox5 (#PIPA5110416; dilution 1:1000) and Col10a1 (#14-9771-80; dilution 1:1000) antibodies were purchased from Fisher Scientific™. The fluorescent secondary antibodies used were mouse and rabbit Alexa Fluor 594 and Alexa Fluor 647, all raised in goat and obtained from Abcam. TEAD1 siRNA was purchased from Santa Cruz Biotechnology. CHX was obtained from Sigma-Aldrich. BrdU labeling, calcein labeling, and EDTA-free cocktail inhibitor tablets, together with the other regents, were obtained from Fisher Scientific™.

Antibodies against Smad2/3 and pSmad2/3 antibodies were obtained from Cell Signaling Technology (CST, USA). Antibodies against TEAD1, TβRII, GFP, flag, Ub, GAPDH, c-Cbl siRNA, and TEAD1 siRNA were ordered from Santa Cruz Biotechnology. The secondary fluorescent antibodies and H&E staining kit were ordered from Abcam. Calcein labeling, osmium tetroxide, and lipofectamine^™ 3000 were purchased from Fisher Scientific^™. Dexamethasone, L-ascorbic acid, and β-glycerophosphate were ordered from Sigma. Plasmids pcDNA3.1-flag and pcDNA3.1-flag-TβRII, GFP-IFT20, HA-Ub were obtained from Addgene.