MDCK cells were plated into six-well plates. Twenty-four hours after plating, the cells were infected with influenza virus. The cells were harvested at 6 h p.i. and washed twice with cold phosphate-buffered saline (PBS) and lysed with a cell lysis buffer (Cell Signaling Technology, Beverly, MA, USA). The cell lysates were analyzed using 12% SDS-polyacrylamide gel electrophoresis (PAGE), blotted onto polyvinylidene fluoride (PVDF) membranes, and then immunoblotted with primary antibodies (Abcam, Cambridge, UK). A horseradish peroxidase (HRP)-conjugated goat anti-rabbit immunoglobulin G (Santa Cruz Biotechnology, Santa Cruz, CA, USA) was used as the secondary antibody. Finally, the membranes were visualized using SuperSignal West Pico Chemiluminescent substrate (Pierce, Rockford, IL, USA). Each reaction was performed in triplicate.
Horseradish peroxidase hrp conjugated goat anti rabbit immunoglobulin g
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Horseradish peroxidase (HRP)-conjugated goat anti-rabbit immunoglobulin G is a secondary antibody used in various immunodetection techniques. It consists of a goat-derived antibody specific to rabbit immunoglobulin G (IgG), conjugated to the enzyme horseradish peroxidase.
Automatically generated - may contain errors
Lab products found in correlation
Cell lysis buffer, by Cell Signaling Technology (1 mentions)
Anti acc, by Cell Signaling Technology (1 mentions)
Glutathione peroxidase gsh px assay kit, by Nanjing Jiancheng (1 mentions)
Malondialdehyde mda assay kit, by Nanjing Jiancheng (1 mentions)
Anti fas, by Cell Signaling Technology (1 mentions)
Micro mitochondrial respiratory chain complex 4 activity assay kit, by Solarbio (1 mentions)
P62 sqstm1, by Merck Group (1 mentions)
Anti cox 4, by Cell Signaling Technology (1 mentions)
Anti β actin, by Cell Signaling Technology (1 mentions)
Hoechst 33258, by Beyotime (1 mentions)
Superoxide dismutase, by Nanjing Jiancheng (1 mentions)
2 protocols using horseradish peroxidase hrp conjugated goat anti rabbit immunoglobulin g
1
Influenza Virus Infection Immunoblotting
2
Cadmium-Induced Metabolic Dysfunction
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The following antibodies and chemicals were used: Cadmium chloride (CdCl2 ), antimicrotubule associated protein 1 light chain 3 betas (LC3B ), and SQSTM1/P62 (Sigma-Aldrich, St. Louis, MO). Melatonin (Shanghai, China). Horseradish peroxidase (HRP )-conjugated goat anti-rabbit immunoglobulin G (Santa Cruz Biotechnology, Santa Cruz, CA). Anti-COX IV, anti-ACC, anti-FAS, anti-β-actin (Cell Signaling Technology, Danvers, MA). Anti-CPT1 and anti-PPAR-α (Proteintech, Wuhan, China). Dihydroethidium (DHE ), Oil Red O Staining Kit, and Hoechst 33258 (Beyotime Biotechnology, Shanghai, China). Micromitochondrial respiratory chain complex I–V activity assay kit (Solarbio, Beijing, China). Malondialdehyde (MDA ) assay kit, glutathione peroxidase (GSH-PX ) assay kit, superoxide dismutase (SOD ), triglyceride assay kit, and total cholesterol assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing. China), and small interfering RNAs (siRNAs ) specific for PPAR-α (RiboBio Corporation, Guangzhou, China).
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