Caco-2 was purchased from DS Pharma Biomedical (Osaka, Japan) and HCT116 was purchased from the American Type Culture Collection. Caco-2 cells were maintained in EMEM (051-07615; Wako) supplemented with 10% fetal bovine serum (FBS; P30-3306; PAN-Biotech GmbH), 5% non-essential amino acids (139-15651; Wako), penicillin, and streptomycin (168-23191; Wako). HCT116 cells were maintained in McCoy's 5A (16600-082; Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS, penicillin, and streptomycin. Human hepatic sinusoidal endothelial cells (HHSECs) were purchased from ScienCell Research Laboratories, Inc. (cat. no. 5000) and maintained with Endothelial Cell Medium (cat. no. 1001; ScienCell Research Laboratories, Inc.) supplemented with 5% FBS and Endothelial Cell Growth Supplement (cat. no. 1052; ScienCell Research Laboratories, Inc.). Cultures were maintained at 37°C in an atmosphere of 95% air and 5% CO2.
Caco 2
Manufactured by Sumitomo Pharma
Sourced in Japan
Caco-2 is an in vitro cell line derived from a human colorectal adenocarcinoma. It is widely used in the pharmaceutical industry as a model for studying the permeability and absorption of drug candidates across the intestinal epithelium.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Penicillin, by Fujifilm (1 mentions)
Streptomycin, by Fujifilm (1 mentions)
Mccoy s 5a, by Thermo Fisher Scientific (1 mentions)
Caco 2, by Fujifilm (1 mentions)
Hct116, by Thermo Fisher Scientific (1 mentions)
Hct116, by American Type Culture Collection (1 mentions)
Endothelial cell growth supplement (ecgs), by ScienCell (1 mentions)
Hhsec, by ScienCell (1 mentions)
Hct116, by Sumitomo Pharma (1 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions)
Sw982, by American Type Culture Collection (1 mentions)
Streptomycin, by Meiji Seika Pharma (1 mentions)
Hacat, by American Type Culture Collection (1 mentions)
Penicillin g, by Meiji Seika Pharma (1 mentions)
3 protocols using caco 2
1
Cultivation of Cell Lines for Research
2
Cultivation of Colonic Epithelial Cell Lines
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Human IEC lines Caco-2 (derived from epithelial colonic adenocarcinoma) and HCT116 (derived from epithelial colonic carcinoma) were purchased from DS Pharma Biomedical and cultured in Eagle’s MEM supplemented with nonessential amino acids and McCoy’s 5a medium, respectively. Another human IEC line SW480 (derived from epithelial colonic adenocarcinoma) was provided by the Cell Resource Center for Biomedical Research Institute of Development, Aging, and Cancer Center at Tohoku University (Miyagi, Japan) and cultured in a 1 : 1 mixture of L15 and DMEM. All media were supplemented with 10% (v/v) FBS, 100 U/mL of penicillin, 100 μg/ml streptomycin, and 5 × 10–5 M 2-mercaptoethanol. Cells were cultured at 37 °C in a humidified incubator with 5% CO2.
3
Maintenance of Various Human Cell Lines
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Immortal human skin keratinocytes (HaCaT, American Type Culture Collection: ATCC, Manassas, VA), human chondrosarcoma (OUMUS, Health Science Research Resources Bank: HSRRB, Osaka, Japan), human colorectal adenocarcinoma (Caco2, DS Pharma Biomedical Co., Osaka, Japan), human alveolar A549 (A549, DS Pharma Biomedical Co., Osaka Japan), human prostate adenocarcinoma (PC3, kindly gifted by Professor Ohya), human synovial sarcoma SW982 (SW982, ATCC), human embryonic kidney 293 cell-line (HEK, HSRRB), and human neuroblastoma IMR32 (IMR32, DS Pharma Biomedical Co.) were maintained in culture medium recommended by the manufacturer. All culture medium was supplemented with 10% heat-inactivated FCS (GIBCO, Waltham, MA), streptomycin (100 μg/mL, Meiji Seika Pharma Co., Ltd., Tokyo, Japan), and penicillin G (100 U/mL, Meiji Seika Pharma Co., Ltd.).
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