Anti bcl 2 clone 100

Manufactured by BioLegend

Anti-BCL-2 (clone 100) is a monoclonal antibody that specifically binds to the BCL-2 protein. BCL-2 is an anti-apoptotic protein that plays a crucial role in regulating cell survival and death. This antibody can be used for the detection and analysis of BCL-2 expression in various cell types and tissues.

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Lab products found in correlation

Countbright absolute counting beads, by Thermo Fisher Scientific (1 mentions) Mouse cd45 clone 30 f11, by BioLegend (1 mentions) Human cd3 clone okt3, by BioLegend (1 mentions) Lsr 2 fortessa cytometer, by BD (1 mentions) Anti human cd8 clone rpa t8, by BioLegend (1 mentions) Anti human cd3 clone sk7, by BioLegend (1 mentions) Anti human cd4 clone a161 a1, by BioLegend (1 mentions) Anti human perforin clone dg9, by BioLegend (1 mentions) Cytofix cytoperm, by BD (1 mentions) Radioimmunoprecipitation assay buffer, by Fujifilm (1 mentions) Anti β actin, by BioLegend (1 mentions) Anti mcl 1, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Bcl-2 (clone 100) Anti-Bcl-2 Bcl-2

3 protocols using anti bcl 2 clone 100

Multiparametric Flow Cytometry for Cell Phenotyping

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Cells were resuspended in FACS staining buffer (PBS + 2% fetal bovine serum) using the following antibodies: human CD3 (clone OKT3, Biolegend), anti-BCL-2 (clone 100, Biolegend), human CD45 (clone 2D1, Biolegend), mouse CD45 (clone 30-F11, Biolegend). CART19 was detected using PE-conjugated anti-CAR19 idiotype antibody (Novartis). To monitor caspase 3/7 activity, CellEvent™ Caspse3/7 Green Read Flow™ reagent was used by following a manufacturing protocol. To determine the absolute cell numbers (tumor or T cells) acquired during flow cytometry, CountBright absolute counting beads (ThermoFisher) were used. Cell viability was established using Live/Dead Aqua or violet fixable staining kit (ThermoFisher), Propidium iodide (PI) and 7-Aminoacctinomycine D (7-AAD), and data were acquired on an LSRII Fortessa Cytometer (BD). Intracellular staining was performed by using fixation/pemeabilization buffer and following manufacturing protocol. All data analysis was performed using FlowJo 9.0 or 10 software (FlowJo, L.L.C., BD, Ashland, OR).

Lee Y.G., Guruprasad P., Ghilardi G., Pajarillo R., Sauter C.T., Patel R., Ballard H.J., Hong S.J., Chun I., Yang N., Amelsberg K.V., Cummins K.D., Svoboda J., Gill S., Chong E.A., North K., Church S.E., Fraietta J.A., Chang W.J., Lacey S.F., Lu X.M., Zhang Y., Whig K., Schultz D.C., Cherry S., Gerson J., Schuster S.J., Porazzi P, & Ruella M. (2022). Modulation of BCL-2 in both T Cells and Tumor Cells to Enhance Chimeric Antigen Receptor T cell Immunotherapy against Cancer. Cancer discovery, 12(10), 2372-2391.

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Multiparametric Flow Cytometry Assay

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At each time point sampled, cells were stained with 1:100 dilutions of the antibodies in PBS with 2% FBS, 2 mM EDTA, fixable viability dye (aqua; Thermo Fisher Scientific, catalog L34966), anti-human CD3 (clone SK7; BioLegend, catalog 344842), anti-human CD4 (clone A161 A1; BioLegend, catalog 357418), anti-human CD8 (clone RPA-T8; BioLegend, catalog 301040), anti-human CD69 (clone FN50; Invitrogen, catalog 47-0699-42), anti-human GZMA (clone CB9; BioLegend, catalog 507215), anti-human GZMB (clone GB11; BioLegend, catalog 515403), anti-human HLA-DR (clone LN3, BioLegend, catalog 327002), anti-human perforin (clone dG9; BioLegend, catalog 308126), and anti-Bcl2 (clone 100; BioLegend, catalog 658709). Cytofix/Cytoperm (BD Biosciences, catalog 554722) was used to fix and permeabilize cells, and 1× Perm Wash buffer was used to dilute intracellular antibodies.

Copertino DC J.r., Holmberg C.S., Weiler J., Ward A.R., Howard J.N., Levinger C., Pang A.P., Corley M.J., Dündar F., Zumbo P., Betel D., Gandhi R.T., McMahon D.K., Bosch R.J., Linden N., Macatangay B.J., Cyktor J.C., Eron J.J., Mellors J.W., Kovacs C., Benko E., Bosque A, & Jones R.B. (2023). The latency-reversing agent HODHBt synergizes with IL-15 to enhance cytotoxic function of HIV-specific T cells. JCI Insight, 8(18), e169028.

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Western Blot Analysis of Cell Signaling Proteins

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Harvested cells were lysed with radio‐immunoprecipitation assay buffer (Fujifilm Wako Pure Chemical) containing cocktails inhibiting protease and phosphatase (Nacalai Tesque). Proteins were electrophoresed on sodium dodecyl sulfate pol yac rylamide gel electrophoresis gels and transferred to polyvinylidene fluoride membranes. After blocking, the membranes were incubated with the following antibodies: anti‐Bcl‐2 (clone 100, #658701; BioLegend); anti‐Bcl‐xL (clone 54H6, #2764; Cell Signaling Technology); anti‐Mcl‐1 (#54535; Cell Signaling Technology); anti‐p21^Cip1/Waf1 (p21) (#2947; Cell Signaling Technology); anti‐p16^Ink4a (p16) (SPC‐1280; StressMarq Biosciences); and anti‐β‐actin (#622102; BioLegend). Thereafter, the membranes were incubated at room temperature for 60 min with goat anti‐rabbit or horse anti‐mouse horseradish peroxidase‐conjugated secondary antibody (#7074 and #7076; Cell Signaling Technology). Bands were visualized by an Amersham ImageQuant 800 Biomolecular Imager (Global Life Sciences Technologies Japan).

Hoque M.M., Iida Y., Kotani H, & Harada M. (2024). Senolysis of gemcitabine‐induced senescent human pancreatic cancer cells. Cancer Reports, 7(4), e2075.

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