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10 protocols using polyethersulfone

1

Functionalized PES Membrane Fabrication

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Polyethersulfone (PES, Mw = 58 k, Ultrason E 6020P, more details are shown in Table S1) was obtained from BASF. Acrylic acid (AA), N-vinylpyrrolidone (NVP), 2,2′-azobis (2-methylpropionitrile) (AIBN), N,N′-methylene bis(acrylamide) (MBA), potassium chloride (KCl), sodium hydroxide (NaOH), sodium chloride (NaCl), ammonium chloride (NH4Cl) and ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA-Na2) were purchased from Aladdin. N,N-dimethylacetamide (DMAc), ethanol (C2H5OH) and normal saline (0.9% NaCl) were ordered from Chengdu Chron Chemicals. Bovine serum albumin (BSA) and bovine serum fibrinogen (BFG) were received from Sigma. Zeolite LTA and mordenite were obtained from Shanghai Saint Chemical New Material. Zeolite Y was ordered from Alfa. Clinoptilolite was received from Guotoushengshi Science & Technology. Permutit was received from Aladdin. Zirconium phosphate (ZrP) was received from Chengdu Huaxia Chemical Reagent. The natural zeolites (clinoptilolite and mordenite) were sourced from China and sifted through a 1000-mesh steel sieve before use.
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2

Synthesis of PETG and Specialized Polymers

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Polyethylene terephthalate glycol (Z-PETG, d = 1.75 mm) was made by Zortrax SA, Olsztyn, Poland.
Polysulfone, polyethersulfone, and polyphenylene sulfone were provided by BASF (Ludwigshafen, Germany).
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3

Polymeric Membrane Fabrication and Characterization

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The following polymeric materials were used in this study. Cellulose acetate (CA) (CH3CO)2O (Mw = 102.09 g/mol) was purchased from Acros Organics (Geel, Belgium). Polyethersulfone (MW = 75,000 g/mol, Tg = 225 °C) Ultra son® E 6020 was supplied by BASF (Ludwigshafen, Germany). Polyvinylpyrrolidone (PVP) (average MW = 40,000) in powder form (C6H9NO)n) was purchased from Sigma–Aldrich (St. Louis, MO, USA) as a pore former and hydrophilic agent. Titanium(IV) TiO2 oxide, Antase 8613-1405 (MW = 79.88) and1–methyl–2–pyrrolidone (NMP) with 99.5% analytical purity as a non–solvent was purchased from Daejung Chemicals (Siheung-si, Korea). Sodium chloride (NaCl) was purchased from Chemsupply (Lahore, Pakistan).
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4

Membrane Fabrication Using Polymer Composites

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Poly (ether sulfone) (Mw = 35,000 g/mol) was obtained from BASF. Dimethylformamide (DMF) and Toluene (99.5%) were employed as solvents and were obtained from Merck chemical Co. MMT (Montmorillonite-K10, surface areas of 220-270 m 2 g À1 ) was provided from Aldrich. The Cation exchange resin (Amberjet 1200D, strongly acidic cation exchanger in Na + form with a capacity of 1.8 meq/L) provided by by Merck Inc., Germany, was also used in membrane preparation. 3-Mercaptopropyl trimethoxysilane (3-MPTS) (95%) was purchased from Sigma. Other chemicals (NaCl, MgCl 2 hexahydrate 99% and CaCl 2 hexahydrate 0.99%) were supplied by Sigma-Aldrich. Throughout the experiments, deionized water (Milli Q, 18.2 MX cm) was used to prepare the solutions.
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5

Polyethersulfone Membrane Fabrication

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Polyethersulfone (PES) (MW= 58000 g.mol-1, ultrason E6020P) and dimethylacetamide (DMAc) as a solvent were purchased from BASF (Germany). Polyvinylpyrrolidone (PVP) (MW= 25000 g mol-1), and ethanol were purchased from Merck (Germany). All chemicals were used without further puri cation. The used milk powder was provided by commercial source (GUIGOZ Growth 3 Formula Milk Powder from 1 year to 3 years), and diesel oil was obtained from Oil Castrol Magnatec (10W-40). For membrane drying step, several lter papers of Whatman (1001-734 Grade 1, size: 46cmX100m) were used.
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6

Polyethersulfone Membrane Fabrication and AMX Characterization

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Polyethersulfone (Mw = 58000 g/mol) provided by BASF Co. was used to prepare membrane casting solution. Pure AMX (Mw = 365.40 g/mol) was prepared from Dana Pharmacy Co. (Tabriz, Iran). Polyvinylpyrrolidone (Mw = 40000 g/mol), 1-Methyl-2-pyrrolidinone (NMP) as a polymer solvent, and Polyethylene glycol hexadecyl ether (Brij®58) as a non-ionic surfactant (HLB = 15.7) were purchased from Sigma Aldrich Co. (USA). N,N-dimethyl-p-phenylenediamine, potassium hexacyanoferrate (iii), NH3, and NaOH were bought from Merck Co. (Germany) to determine AMX content in feed and permeate flow. Molecular structure of PES, Brij®58, PVP, NMP, and AMX is shown in Figure 1.

Chemical structure of PES, PVP 40, NMP, Brij®58 and AMX.

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7

Fabrication of Polymeric Membrane Filters

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Polyethersulfone (PES, Mw = 45 000) and polysulfone (PSF) were acquired from BASF Co. Ltd. (Germany). N,N-Dimethyl acetamide (DMAc), diethylene glycol (DEG), chloroform, trimethylsilyl chlorosulfonate (Aldrich) and sodium methoxide were purchased by Shanghai Chemical Reagent Co. Ltd. Bovine serum albumin (BSA, Mw = 67 000) was supplied by Shanghai Lianguan Biochemical Engineering Co. Ltd. Pure water was self-made.
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8

Fabrication and Characterization of PES-PVP Membranes

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Polyethersulfone (PES, MW 58 kDa) and polyvinylpyrrolidone (PVP, MW 360 kDa) were purchased from Basf, Ludwigshafen am Rhein, Germany and Sigma-Aldrich, St. Louis, MI, USA, respectively. The common solvent for both polymers was N, N-dimethylacetamide (DMAc, 99.5%), purchased from Tedia, Fairfield, Ohio, EUA. For microbiological experiments, deionized water was supplied by a Milli-Q apparatus (Merck KGaA, Darmstadt, Germany). Yeast extract, meat peptone, and agar were purchased from Kasvi, São José do Pinhais, Brazil. Magnesium sulfate, potassium phosphate monobasic, potassium phosphate dibasic, and glycerol were purchased from Vetec, Duque de Caxias, Brazil. These reagents were used as culture medium. PES and PVP were dried at 60 °C overnight before being used and the other reagents were used as received.
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9

Fabrication of Polyethersulfone-Based Membranes

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Polyethersulfone (BASF Co., Germany) was used after drying at 120 °C for at least 24 h. Graphite power (325 mesh) was purchased from Alfa Aesar, USA. Nafion solution (D2021, dispersion alcohol-based 1100 EW at 20 wt%, Fuel Cell Store, USA) was employed as the pore-filling ionomer. Deionized water (DI) was obtained from Milli-Q System (Millipore, USA). Dimethylacetamide (DMAc), hydrochloric acid (HCl, mass fraction of 35%), sulfuric acid (H2SO4, mass fraction of 98%), potassium permanganate (KMnO4), hydrogen peroxide (H2O2, mass fraction of 30%), sodium chloride (NaCl), and sodium hydroxide (NaOH) were provided by Daejung Chemical, Republic of Korea. All chemicals were used without further purification.
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10

Characterization of Crude Oil Emulsions

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The materials used in this study were used as received because they were all of analytical grade. Polyethersulfone (PES) and tagged Ultrason E6020P was supplied by BASF (Germany). Thiourea (CH4N2S) was acquired from Advent ChemBio PVT Limited (India), whereas ammonium molybdate ((NH4)6Mo7O24·4H2O) was bought from Suv Chem Laboratory Chemicals (India). Polyvinylpyrrolidone (PVP) as well as N-methyl-2-pyrrolidone (NMP) were purchased from Loba Chemie PVT Limited (India). Triethylenglycol (TEG) of 98% purity was purchased from Arcos (Belgium). Crude oil collected from a real sample obtained locally from an Egypt-based petroleum company was utilized to synthesize emulsions of crude oil in water at three concentrations (100 mg L−1, 1000 mg L−1, and 10 000 mg L−1). The viscosity (cSt), specific gravity, API gravity, sulphur concentration (%), density (kg m−3) and P.P (F0) of the crude oil used in this study were 45, 0.915, 23, 3, 0.945 and 40, respectively. All emulsions were made fresh prior to use to avoid the coalescence of oil droplets.
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