Anti mouse and anti rabbit conjugated horseradish peroxidase hrp antibodies

Soya-cerebrosides (Fig. 1A) of >98% purity were synthesized at the College of Pharmacy, School of Pharmacy, China Medical University, Taichung, Taiwan (The detail synthesis procedures and purified quality was provided as described previously21 (link)). Soya-cerebroside was dissolved in DMSO for intraperitoneal (IP) injection. A miR-432 inhibitor, Control miRNA and TRIzol were from Life Technologies (Carlsbad, CA). Rabbit polyclonal antibody specific for MCP-1, MCP-1 enzyme-linked immunosorbent assay (ELISA) kit and human recombinant IL-1β were purchased from R&D Systems (Minneapolis, MN, USA), anti-phospho AMPK (Thr172) and phospho AKT (Ser473) from Cell Signaling (Danvers, MA, USA). Mouse monoclonal CD68 was purchased from Novus (Novus, Littleton, USA) and Ara A (ATP mimetic), Compound C (AMPK kinase inhibitor), and AKTi (AKT1/2 kinase inhibitor) from Enzo Biochem, Inc. (Enzo, New York, NY). Anti-AMPK, anti-AKT, and anti-mouse and anti-rabbit conjugated horseradish peroxidase (HRP) antibodies were from Santa Cruz (Santa Cruz, CA, USA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA).

The reagent Trizol, Lipofectamine 2000 transfection reagent, miR-519b mimic, and control miRNA were purchased from Life Technologies (Carlsbad, CA). Recombinant human TGF-β1 and the TGF-β1 ELISA kit were bought from PeproTech (Rocky Hill, NJ, USA). The antibodies against phospho PLCγ and phospho PKCα were the products from the Cell Signaling (Danvers, MA, USA). U73122, Gö6976, and GF109203X were bought from Enzo Biochem, Inc. (Enzo, New York, NY). Anti-PLCγ, anti-PKCα, anti-HO-1, anti-mouse and anti-rabbit conjugated horseradish peroxidase (HRP) antibodies were the products from Santa Cruz (Santa Cruz, CA, USA). The small interfering RNAs (siRNAs) against PLCγ, PKCα as well as the control siRNAs were the products from the Dharmacon Research (Lafayette, CO, USA). The pertinent chemicals not mentioned above were purchased from the Sigma-Aldrich (St. Louis, MO, USA).