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4 6 diamidino 2 phenylindole dapi

Manufactured by Bioworld Technology
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4',6-diamidino-2-phenylindole (DAPI) is a fluorescent stain that binds strongly to adenine-thymine (A-T) rich regions in DNA. It is commonly used as a nuclear counterstain in fluorescence microscopy to visualize the cell nucleus.

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Lab products found in correlation

Facsaria 3, by BD (1 mentions) Goat anti rabbit alexa fluor 594, by Abcam (1 mentions) Anti γh2ax antibody, by Cell Signaling Technology (1 mentions) Fluorescence microscope, by Leica (1 mentions) Goat serum, by Zhongshan Golden Bridge Biotechnology (1 mentions)

2 protocols using 4 6 diamidino 2 phenylindole dapi

1

Cell Cycle Analysis by Flow Cytometry

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The cell cycle analysis was performed with Flow cytometry. The cells were harvested at 36 h post transfection of Setdb1 siRNA or control siRNA. After being fixed in 70% cold ethanol, the cells were incubated with RNase and finally stained with 4′,6-diamidino-2-phenylindole (DAPI, Bioworld). DNA content was analyzed by Flow cytometry (BD FACSAriaTM III, United States). The data were analyzed with ModFit LT 5.0.
Li X., Chen X., Liu Y., Zhang P., Zheng Y, & Zeng W. (2020). The Histone Methyltransferase SETDB1 Modulates Survival of Spermatogonial Stem/Progenitor Cells Through NADPH Oxidase. Frontiers in Genetics, 11, 997.
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2

Immunofluorescence Assay for γH2A.X

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Briefly, the cells on slides were immobilized using 4% paraformaldehyde and permeabilized using 0.1% Triton X-100 in PBS. Then, the cells were blocked using goat serum (Zhongshan Golden Bridge, China) and incubated overnight at 4°C with anti-γH2A.X antibody (Cell Signaling, MA, USA, 1 : 500). After washing, the cells were incubated with goat anti-rabbit Alexa Fluor 594 (Abcam, UK, 1 : 1000). The cells were then counterstained with 4′,6-diamidino-2-phenylindole (DAPI, Bioworld, China, 1 : 2000) for 5 min. The slides were imaged using a fluorescence microscope (Leica, Germany).
Li Z., Liu L., Yang Y., Zheng H., Cai Y., Ma Y., Gu R., Xu K., Zhang R, & Xu P. (2022). Metformin Ameliorates Senescence of Adipose-Derived Mesenchymal Stem Cells and Attenuates Osteoarthritis Progression via the AMPK-Dependent Autophagy Pathway. Oxidative Medicine and Cellular Longevity, 2022, 4620254.
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