All the animal experiments were reviewed and approved by the Animal Welfare Committee of University of Texas Health Science Center at Houston (Animal protocol number: AWC-18-0057). Mice were housed in animal facility under 12-hour light-dark cycle at 22 ± 1°C. The mice had ad libitum access to water and regular chow diet, unless indicated otherwise. C57BL/6J (Stock 000664) and Adiponectin-Cre (Stock 010803) were purchased from The Jackson Laboratory. Drp1 floxed mouse (Drp1flx/flx) was described previously.3 (link) Adipose tissues-specific DRP1 knockout (Adipo-Drp1flx/flx) mice were generated by crossing the lines of Adiponectin-Cre and Drp1flx/flx. For the cold exposure assay, male mice were housed individually in 6°C chamber without bedding and diets. The rectal temperatures were measured every 2 hours during the process.
Adiponectin cre stock 010803
Manufactured by Jackson ImmunoResearch
Adiponectin-Cre (Stock 010803) is a transgenic mouse line that expresses Cre recombinase under the control of the adiponectin promoter. Adiponectin is a protein secreted by adipose tissue and plays a role in regulating glucose and lipid metabolism.
Automatically generated - may contain errors
2 protocols using adiponectin cre stock 010803
1
Adipose-specific DRP1 Knockout Mice Cold Tolerance
2
Conditional knockout mice for metabolic studies
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All of the animal studies were reviewed and approved by the Animal Welfare Committee (AWC) of University of Texas Health Science Center at Houston (Animal protocol number: AWC-21-0019). Wild-type (WT) C57BL/6J (Stock 000664) and Adiponectin-Cre (Stock 010803) mice were purchased from Jackson Laboratories. The Ces1d-floxed (Ces1dflx/flx) mouse was generated by Dr. Grant A Mitchell from the Ste-Justine Hospital in Montreal, Canada, and crossed onto C57BL/6J background (18 (link)). 8-wk-old male FKO and their littermate floxed control (from here on referred to as WT) mice were used for all the experiments. Mice were housed in the animal facility (22°C ± 1°C) on a 12-h light/dark cycle with ad libitum access to water and regular chow diet, unless indicated otherwise. For the HFD challenge, the age matched male WT and FKO mice were fed with the HFD (containing 60% calories from fat; Bio-Serv) for 14 or 25 wk. The total body mass, fat mass and lean mass were measured by EchoMRI-100T (Echo Medical Systems).
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