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Daidzein

Manufactured by ChromaDex
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Daidzein is a type of isoflavone, which is a class of phytoestrogens found in various plants. Daidzein is a naturally occurring compound that can be isolated and purified for use in laboratory settings. It serves as a useful chemical standard and reference material for analytical and research applications.

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Lab products found in correlation

Genistein, by ChromaDex (3 mentions) Daidzin, by ChromaDex (2 mentions) Genistin, by ChromaDex (1 mentions) β carotene, by Merck Group (1 mentions) Formononetin, by Merck Group (1 mentions) 2 2 diphenyl 1 picrylhydrazyl radical dpph, by Merck Group (1 mentions) Biochanin a, by Merck Group (1 mentions) Mecamylamine hydrochloride, by Merck Group (1 mentions) Isoquercitrin, by ChromaDex (1 mentions) Astragalin, by ChromaDex (1 mentions) Delphinidin, by ChromaDex (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Hank s balanced salt solution hbss, by Thermo Fisher Scientific (1 mentions) Baicalein, by ChromaDex (1 mentions) Isorhamnetin, by ChromaDex (1 mentions) Lipopolysaccharide lps from escherichia coli serotype 026 b6, by Merck Group (1 mentions) Malvidin, by ChromaDex (1 mentions) Methanol, by Merck Group (1 mentions) Nicotine, by Merck Group (1 mentions) Griess reagent system, by Promega (1 mentions)

3 protocols using daidzein

1

Analytical Extraction of Bioactive Compounds

Cited 1 time
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Methanol, methyl tert-butyl ether, acetonitrile, water, hexane, acetone, formic acid and ammonium were of HPLC grade and purchased from Fisher Scientific. Beta-carotene was purchased from Sigma Aldrich, soy isoflavones (genistein, genistin, daidzein, daidzin, glycitein, and glycitin) were from ChromaDex, and lycopene was isolated and purified from tomato52 (link).
Mukherjee D., DiVincenzo M.J., Torok M., Choueiry F., Kumar R.J., Deems A., Miller J.L., Hinton A., Geraghty C., Maranon J.A., Kulp S.K., Coss C., Carson WE I.I.I., Conwell D.L., Hart P.A., Cooperstone J.L, & Mace T.A. (2020). Soy-tomato enriched diet reduces inflammation and disease severity in a pre-clinical model of chronic pancreatitis. Scientific Reports, 10, 21824.
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2

Isoflavone Extraction and Analysis

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Solvents used for extraction and HPLC analysis included the following: ethanol 96% (Zorka Pharm, Šabac, Serbia), hydrochloric acid 35% (POCH, Gliwice, Poland), methanol—HPLC grade, min 99.8% (J.T. Baker, Phillipsburg, NJ, USA), distilled water (TKA water purification system type 05.30C 7-DEN, Department of Pharmacy, Faculty of Medicine Novi Sad), sulfuric acid p.a. (RTB, Bor, Serbia) and acetonitrile—HPLC grade (J.T. Baker, Phillipsburg, NJ, USA). During total phenolic content determination, anhydrous sodium carbonate, min 99.5% (Sinex laboratory, Belgrade, Serbia), Folin–Ciocalteu phenolic reagent (Fluka Biochemika, Buchs, Switzerland) and gallic acid, 99% (Alfa Aesar Lancaster Synthesis, Ward Mill, MA, USA) were used. Antioxidant potential was tested with 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical (Sigma Aldrich, Steinheim, Germany). Standard isoflavone substances were daidzein, genistein (ChromaDex, Los Angeles, CA, USA), formononetin and biochanin A (Sigma-Aldrich).
Mikulić M., Atanacković Krstonošić M., Kladar N., Vasiljević S., Katanski S., Mamlić Z., Rakić D, & Cvejić J. (2023). Phytochemical Composition of Different Red Clover Genotypes Based on Plant Part and Genetic Traits. Foods, 13(1), 103.
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3

Neurochemical Signaling Pathway Analysis

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Methanol, hexane, chloroform, ethyl acetate, butanol, lipopolysaccharide (LPS) from Escherichia coli serotype 026:B6 (Lot# 021M4072V), (-)-nicotine, methyllycaconitine citrate salt hydrate (MLA) from Delphinium brownii seeds, mecamylamine hydrochloride and 7-hydroxy-3H-phenoxazin-3-one-10-oxide sodium salt (resazurin) were purchased from Sigma-Aldrich (St Louis, MO, USA). Astragalin, baicalein, catechin, daidzin, daidzein, delphinidin, genistein, isoquercitrin, isorhamnetin, malvidin, petunidin-3-glycoside, quercetagetin, quercetin, rhamnetin, sakuranetin, spiraeoside and tamarixetin (Fig. 1) were purchased from Chromadex (Irvine, CA, USA). [3H]-MLA (∼60Ci/mmol), [3H]-cytisine (∼16Ci/mmol) and [3H]-epibatidine (∼30Ci/mmol) were purchased from American Radiolabeled Chemicals, Inc. (St. Louis, MO, USA). Antibiotics (10000U/mL penicillin and 10000ug/mL streptomycin), 2.5% trypsin (10×), fetal bovine serum (FBS), Dulbeccos' Modified Eagle Medium (DMEM), DMEM:Nutrient mixture F-12 (DMEM/F-12) and Hanks Balanced Salt Solution (HBSS) were purchased from Life Technologies Corporation (Grand Island, NY, USA). Griess reagent system was purchased from Promega Corporation (Madison, WI, USA). TNF-alpha ELISA Ready-SET-Go!® was purchased from eBioscience Inc. (San Diego, CA, USA).
Lutz J.A., Kulshrestha M., Rogers D.T, & Littleton J.M. (2014). A nicotinic receptor-mediated anti-inflammatory effect of the flavonoid rhamnetin in BV2 microglia. Fitoterapia, 98, 11-21.
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