Bromopyruvic acid

Manufactured by Merck Group

Sourced in United States

Bromopyruvic acid is a chemical compound that is used in laboratory settings. It is a colorless, crystalline solid with the molecular formula C₃H₃BrO₃. Bromopyruvic acid is commonly used as a reagent in various biochemical and organic synthesis applications.

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3-bromopyruvic acid (3 citations)

4 protocols using bromopyruvic acid

Pharmacological Screening Library Compounds

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Rifampicin was purchased from AppliChem. Bromopyruvic acid, dexamethasone, gentamicin, staurosporine, and thalidomide were purchased from Sigma-Aldrich. Cyclosporin A, doramapimod, moxifloxacin, necrostatin-1, necrostatin-5, and tacrolimus (FK506) were obtained from Cayman Chemicals. BMS-582949, pamapimod, and Z-VAD-FMK were purchased from Selleckchem. Puromycin dihydrochloride was from Carl Roth (Germany). GR agonist BI653048 was provided by the opnMe platform (Boehringer Ingelheim). The Prestwick chemical library was used for high-throughput screening.

Gräb J., Suárez I., van Gumpel E., Winter S., Schreiber F., Esser A., Hölscher C., Fritsch M., Herb M., Schramm M., Wachsmuth L., Pallasch C., Pasparakis M., Kashkar H, & Rybniker J. (2019). Corticosteroids inhibit Mycobacterium tuberculosis-induced necrotic host cell death by abrogating mitochondrial membrane permeability transition. Nature Communications, 10, 688.

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Enzymatic Characterization of Lysozyme

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Lysozyme (Lyz) from chicken egg white (about 100,000 U/mg), L-cysteine (Cys), L-cystine, L-glutathione (GSH), oxidized glutathione (GSSG), cysteamine, cystamine, 1-chloro-2,4-dinitrobenzene (CDNB), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), 4-chloro-7-nitrobenzofurazane (NBD-Cl), dithiotreitol (DTT), bromopyruvic acid, lysozyme activity kit, pepsin A and all other reagents were from SIGMA-Aldrich (St. Louis, Mo, USA).

Bocedi A., Cattani G., Martelli C., Cozzolino F., Castagnola M., Pucci P, & Ricci G. (2018). The extreme hyper-reactivity of Cys94 in lysozyme avoids its amorphous aggregation. Scientific Reports, 8, 16050.

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Bovine α-Chymotrypsinogen A Characterization

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α-Chymotrypsinogen A (ChTg) from bovine pancreas. The protein was tested for purity by using an FPLC system (Amersham-Pharmacia, Sweden) equipped with a column Superose-12 (data not shown). Bromopyruvic acid (BrP), cystamine, L-cysteine (Cys), 1-chloro-2,4-dinitrobenzene (CDNB), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), dithiothreitol (DTT), ethylendiamminotetreaacetic acid (EDTA), L-glutathione (GSH), oxidized glutathione (GSSG), homocystine, N-acetyl-L-tryptophanamide (NATA), 4-chloro-7-nitrobenzofurazane (NBD-Cl), and all other reagents were from Sigma-Aldrich (St. Louis, Mo, USA). GSH solutions were freshly prepared and the amount of GSSG was less than 1% as assayed by standard analytical procedures.

Bocedi A., Gambardella G., Cattani G., Bartolucci S., Limauro D., Pedone E., Iavarone F., Castagnola M, & Ricci G. (2020). Ultra-rapid glutathionylation of chymotrypsinogen in its molten globule-like conformation: A comparison to archaeal proteins. Scientific Reports, 10, 8943.

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Investigating RNase A Enzymatic Activity

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Ribonuclease A (RNase) from bovine pancreas (Type XII-A, 75–125 Kunitz units/mg protein), bromopyruvic acid, copper sulfate, dithiothreitol (DTT), ethylendiamminotetreaacetic acid (EDTA), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), L-glutathione (GSH), oxidized glutathione (GSSG), β-mercaptoethanol (β-ME), Ribonuclease A activity kit and all other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA).
The GSH solutions were prepared immediately before use and the GSSG concentration was less than 1% as assayed by standard analytical procedures.
All organic solvents were of LC-MS grade. Acetonitrile (ACN), methanol (MeOH), formic acid (FA) and water were from Merck (Darmstadt, Germany). Trypsin (Gold MS Grade) was from Promega (Madison, WI, USA).

Gambardella G., Notari S., Cavaterra D., Iavarone F., Castagnola M., Bocedi A, & Ricci G. (2022). The Anfinsen Dogma: Intriguing Details Sixty-Five Years Later. International Journal of Molecular Sciences, 23(14), 7759.

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