Lamda 750s

The morphologies of PSi films were characterized using a desktop scanning electron microscopy (SEM) (Phenom G2 Pro, Phenom-World, Eindhoven, The Netherlands) and a Field Emission-SEM (FE-SEM) (ZEISS-Ultra55, Carl Zeiss AG, Oberkochen, Germany). The reflection spectra of the PSi films before and after transferred onto the tape were both recorded in the wavelength range of 450–700 nm by using a spectrometer (USB 2000+, Ocean Optics, Dunedin, FL, USA) and UV-VIS-NIR spectrophotometer (Lamda 750s, PerkinElmer Inc., Shelton, CT, USA).

1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (DOPE-mPEG2000) was purchased from Avanti Polar Lipids. A total of 20% Intralipid emulsion was purchased from Sigma-Aldrich. Analytical grade solvents including petroleum ether, CH₂Cl₂, and ethyl acetate were purchased from Titan Scientific. All other chemicals were purchased from TCI and J&K without further purification unless otherwise noted. All ¹H-NMR and ¹³C-NMR spectra were acquired on a Bruker AV-400 spectrometer. Chemicals shifts are referenced to the residue solvent peaks and given in ppm. MALDI-TOF MS analyses were performed in positive reflection mode on a 5800 proteomic analyzer (Applied Biosystems, Framingham, MA, USA) with a Nd: YAG laser. UV-Vis-NIR absorption spectra were recorded on Lamda750S PerkinElmer. NIR-II fluorescence spectra were recorded on Edinburgh Fluorescence Spectrometer FLS980 instrument with external 808 (MDL-III-2W), 915 (MDL-H-5W), or 1064 nm (MIL-III-1.5 W) semiconductor laser (Changchun New Industries Optoelectronics Tech. Co., Ltd.) as excitation source. In vivo NIR-II images were taken using NIRvana CCD camera (Princeton Instruments Inc.). Dynamic light scattering were carried out on a Malvern Zetasizer 3600 (Malvern Instruments).