S monovette edta k2 anticoagulant tubes

The blood samples of patients with GBM were collected from the median cubital vein in the morning between 7.00 and 9.00 on the day of admission to hospital (one day before the surgical resection) and before the introduction of steroid therapy. Blood samples were collected in S-Monovette-EDTA K2 anticoagulant tubes (Sarstedt AG & Co. KG, Nümbrecht, Germany) and immediately subjected to haematology analysis in the laboratory of the University Hospital in Zielona Gora. For further experiments, blood samples were centrifuged at 3000 rpm for 10 min and aliquots of plasma were stored at −80 °C.

Peripheral blood was collected from the median cubital vein in participants who were fasting and physically inactive for at least 48 h prior to blood sampling. Blood samples collected in S-Monovette-EDTA K2 anticoagulant tubes (Sarstedt, Germany) were subjected for hematology analysis, while blood collected in S-Monovette clotting activator tubes (Sarstedt, Germany) was applied for biochemical analysis. Prior to centrifugation, blood collected in a clotting activator tube was allowed to clot for 45 min. After 45 min, the blood collected was centrifuged for 10 min at 3000 g and + 8°C. Serum aliquots were stored in a low-temperature freezer that maintains a temperature at −80°C until biochemical analysis and inflammatory markers were performed.