Goat anti rabbit igg horseradish peroxidase hrp linked antibody

Mouse monoclonal antibody against RPS19 was from Abnova (Taiwan). Rabbit polyclonal antibody to RPL5 was from Abcam (Cambridge, MA). Rabbit polyclonal antibody, raised against a full-length human p53 fusion protein, was from Cell Signaling Technology (Danvers, MA). Mouse monoclonal antibody against β-Actin was from Santa Cruz Biotechnology (California). Goat anti-rabbit IgG horseradish peroxidase [HRP]-linked antibody was from Cell Signaling Technology. Goat anti-mouse IgG HRP-linked secondary antibody was from Santa Cruz.

The compounds used in this study were purchased as follows. BTZ was from FUJIFILM Wako Pure Chemical. MLN9708 (IXA citrate), delanzomib, and z-FA-FMK were from Cayman Chemical. MDL-28170 was from Merck. Proteasome inhibitors MG132, MG115, and PSI were from the PEPTIDE Institute. Carfilzomib was from Adipogen. z-VAD-FMK was from Bachem. These compounds were separately dissolved in DMSO (Nacalai tesque) at 10 mM and diluted with Dulbecco’s Modified Eagle’s Medium (FUJIFILM Wako Pure Chemical).
The antibodies used in this study were purchased as follows. Rabbit anti-human cleaved caspase-3 (Asp175) polyclonal antibody (#9661), rabbit anti-human caspase-7 antibody (#9492), rabbit anti-human cleaved PARP (Asp214) polyclonal antibody (#9541), goat anti-rabbit IgG horseradish peroxidase (HRP)-linked antibody (#7074), and horse anti-mouse IgG HRP-linked antibody (#7076) were from Cell Signaling Technology. Mouse anti-β-actin (C4) monoclonal antibody (sc-47778) was from Santa Cruz Biotechnology.