Cf770 antibody

Chemical and reagents. Penicillin, streptomycin, trypsin, PBS, RNAse, DMSO and Triton X-100 were purchased from Sigma-Aldrich. The blocking solution (MAXblock Blocking Medium) was purchased from Active Motif (Belgium). CF770 antibody (goat anti-rabbit and anti-mouse antibodies) and RedDot2 were purchase from Biotium (Hayward, CA, USA). Mouse anti-human CD59 (Protectin) APC monoclonal antibody was purchased from Affymetrix (eBioscience, USA). FLAER: Alexa fluor 488 proaerolysin was purchase from Cedarlane (CA).
Culture of HepG2 cells. HepG2 human hepatoblastoma cells (ATCC N° HB-8065) were routinely grown in 75-cm 2 culture flasks in αMEM medium supplemented with 10% FBS, 100 U mL -1 Penicillin and 100 µg mL -1 streptomycin. Cultures were maintained in a humidified atmosphere with 5% CO2 at 37 °C and the medium was refreshed every 2-3 days.

AFB1, AFB2, AFG1, AFG2 and AFM1; ST, OMST, and CPA were purchased from Sigma-Aldrich (Saint Quentin Fallavier, France). Averantin was provided by BioViotica Naturstoffe GmbH (Dransfeld, Germany). AFL was purchased from Fermenteck (Jerusalem, Israel). NOR and VERA were purified from wheat inoculated with Aspergillus toxigenic stocks, as described below. Almost all stock solutions of the compounds were prepared in 100% dimethyl sulfoxide (DMSO). From the stocks, 10-fold dilution series were prepared. Penicillin, strepto-mycin, trypsin, PBS, RNAse A, and Triton X-100 were purchased from Sigma-Aldrich (Saint Quentin Fallavier, France). The phosphatase inhibitor cocktail tablets ("PHOSSTOP") were purchased from Roche (Meylan, France), and the blocking solution (MAXblock Blocking Medium) was purchased from Active Motif (Rixensart, Belgium). CF770 antibody and RedDot2 were purchased from Biotium (Hayward, California, USA). All solvents used in the extraction and high performance liquid chromatography (HPLC) analysis were analytical grade and purchased from Fisher Scientific (Illkirch, France). Water for HPLC-DAD and molecular biology procedures was purified by using a Millipore MilliQ purification system.

All stock solutions of the compounds were prepared in 100 % dimethyl sulfoxide (DMSO). From the stocks, 10-fold dilution series were prepared for all the compounds, except cytotoxic compounds for which threefold dilution series were prepared. All the compounds were of analytical grade and were purchased from Sigma-Aldrich (Saint Quentin Fallavier, France), excepted for alisertib, tozasertib and volasertib, which were purchased from Selleckchem (Euromedex, France). Penicillin, streptomycin, trypsin, PBS, RNAse A and Triton X-100 were purchased from Sigma-Aldrich. The phosphatase inhibitor cocktail tablets ("PHOSSTOP") were purchased from Roche, and the blocking solution (MAXblock Blocking Medium) was purchased from Active Motif (Belgium). CF770 antibody (goat antibody anti-rabbit and anti-mouse) and RedDot2 were purchased from Biotium (Hayward, CA, USA).