For western blotting the following antibodies were used: total LRRK2 [MJFF2 (c41-2)] (Abcam, Cambridge, UK, Cat# ab133474, 1:300), phospho-S935 LRRK2 (Abcam, Cambridge, UK, Cat# ab133450, 1:500), phospho-S1292 LRRK2 (Abcam, Cambridge, UK, Cat# ab203181, 1:300), total Rab10 (Abcam, Cambridge, UK, Cat# ab104859, 1:500), phospho-T73 Rab10 (Abcam, Cambridge, UK, Cat# ab230261, 1:400), tyrosine hydroxylase (Millipore, Burlington, MA, USA Cat# AB152, 1:10000), DARPP32 (Millipore, Burlington, MA, USA Cat# AB10518, 1:10000) and β-actin (Sigma-Aldrich, St. Louis, MO, USA Cat# A2066, 1:10000). For immunofluorescence: β-tubulin-III (Sigma-Aldrich, St. Louis, MO, USA, Cat# T8578, 1:1000); total LRRK2 [MJFF2 (c41-2)] (Abcam, Cambridge, UK, Cat# ab133474, 1:200), phospho-S935 LRRK2 [UDD2 10(12)] (Abcam, Cambridge, UK, Cat# ab172382, 1:200), GLT1 (EMD Millipore, Burlington, MA, USA Cat# AB1783, 1:400), GFAP (Dako-Agilent, Santa Clara, CA, USA, Cat# Z0334, 1:400) CD11b [M1/70] (eBioscience™ from Thermo Fisher Scientific, Waltham, MA, USA, Cat# 14-0112-82, 1:200).
Iannotta L., Biosa A., Kluss J.H., Tombesi G., Kaganovich A., Cogo S., Plotegher N., Civiero L., Lobbestael E., Baekelandt V., Cookson M.R, & Greggio E. (2020). Divergent Effects of G2019S and R1441C LRRK2 Mutations on LRRK2 and Rab10 Phosphorylations in Mouse Tissues. Cells, 9(11), 2344.
+ Open protocol