Vascular tissue samples were dissected from regular wild type C57Bl6
mice, and rapidly embedded for frozen cross-sectioning. Sections were cut at 5
μm and mounted onto glass slides. Afterwards, Clock components were
immunohistochemically detected. Briefly, the indirect avidin biotin-horseradish
peroxidase visualization method was used (ABC Standard and Elite, Vector Red,
Vector Laboratories, Burlingame, USA). Samples were incubated with the detection
primary antibody at the manufacturer’s recommended concentration.
mice, and rapidly embedded for frozen cross-sectioning. Sections were cut at 5
μm and mounted onto glass slides. Afterwards, Clock components were
immunohistochemically detected. Briefly, the indirect avidin biotin-horseradish
peroxidase visualization method was used (ABC Standard and Elite, Vector Red,
Vector Laboratories, Burlingame, USA). Samples were incubated with the detection
primary antibody at the manufacturer’s recommended concentration.