At the 14 days, administered (Cohort 2) i.p. with 3% Evans Blue (EB; Sigma-Aldrich, USA) as described previously (39 (link)). Four hours after post-injection, the minks were sacrificed. The brains were harvested and fixed in 4% formalin (BBI Life Sciences Corporation, China). Frozen minks brain slabs (6 μm) are kept at cryogenic temperatures, and sections were examined in OLYMPUS Digital Pathology (OLYMPUS, Japan). ImageJ software was used as the integrated density analysis tool to measure extravascular accumulations of EB. Three micrographs per individual were performed for the photomicrographs.
Digital pathology
Manufactured by Olympus
Sourced in Japan
Digital Pathology is a system that enables the digitization and analysis of pathological samples. It captures high-resolution images of tissue samples and stores them in a digital format, allowing for remote access and collaboration among healthcare professionals.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using digital pathology
1
Measuring Brain Evans Blue Extravasation
2
Histological Analysis of Tibialis Anterior Muscle
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fresh isolated tibialis anterior muscle samples were mounted on 4% paraformaldehyde. The sections of muscle tissues (10 μm) were subjected to hematoxylin and eosin staining using a commercial kit (Solarbio, China). The sections were detected using OLYMPUS Digital Pathology (OLYMPUS, Japan). Eight micrographs per field were performed for the photomicrographs.
3
Quantifying Vascular Permeability in Mink Brains
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At the 14 days, administered (Cohort 2) i.p. with 3% Evans Blue (EB; Sigma-Aldrich, USA) as described previously [39] . Four hours after post-injection, the minks were sacri ced. The brains were harvested and xed in 4% formalin (BBI Life Sciences Corporation, China). Frozen minks brain slabs (6 µm) are kept at cryogenic temperatures and sections were examined in OLYMPUS Digital Pathology (OLYMPUS, Japan). "Image J" was used as the integrated density analysis tool to measure extravascular accumulations of EB. Three micrographs per individual were performed for the photomicrographs.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!