Log In Sign up
The largest database of trusted experimental protocols

Fluorescein isothiocyanate or allophycocyanin labeled annexin 5

Manufactured by BD
Visit Supplier
Sourced in United States

Fluorescein isothiocyanate- or allophycocyanin-labeled annexin V is a reagent used in flow cytometry and microscopy applications. Annexin V is a calcium-dependent phospholipid-binding protein that has a high affinity for phosphatidylserine, which is exposed on the surface of apoptotic cells. The fluorescent labels, either fluorescein isothiocyanate or allophycocyanin, allow the detection and visualization of apoptotic cells.

Automatically generated - may contain errors

Lab products found in correlation

Facscalibur flow cytometer, by BD (2 mentions) Propidium iodide, by Roche (1 mentions)

Spelling variants of this product

Annexin V (870 citations) Annexin V-fluorescein isothiocyanate (148 citations) Annexin V-fluorescein (21 citations) Annexin V-allophycocyanin (15 citations) Fluorescein isothiocyanate-labeled annexin V (2 citations)

2 protocols using fluorescein isothiocyanate or allophycocyanin labeled annexin 5

1

Apoptosis Assessment by Annexin V-PI

Check if the same lab product or an alternative is used in the 5 most similar protocols
The cells were stained with fluorescein isothiocyanate- or allophycocyanin-labeled annexin V (BD Biosciences, Franklin Lakes, New Jersey, USA) and propidium iodide (PI; Hoffman-La Roche, Basel, Switzerland), according to the manufacturer’s instructions. The treated cells were analyzed using a FACSCalibur flow cytometer (BD Biosciences). The annexin V+ PI+ cell fraction indicated apoptotic cells.
Tsuma-Kaneko M., Sawanobori M., Kawakami S., Uno T., Nakamura Y., Onizuka M., Ando K, & Kawada H. (2018). Iron removal enhances vitamin C-induced apoptosis and growth inhibition of K-562 leukemic cells. Scientific Reports, 8, 17377.
+ Open protocol
+ Expand
2

Annexin V and PI Staining for Apoptosis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Detection of apoptotic cells was done with fluorescein isothiocyanate- or allophycocyanin-labeled annexin V (BD Biosciences, Franklin Lakes, NJ, USA) and PI (Hoffman-La Roche, Basel, Switzerland), as described [53 (link)]. Apoptotic cells were analyzed on a BD FACSCalibur flow cytometer (BD Biosciences). Early apoptotic cells were stained with annexin V but not PI, whereas late apoptotic or necrotic cells were stained with both annexin V and PI.
Suzuki R., Kitamura Y., Nakamura Y., Akashi H., Ogawa Y., Kawada H, & Ando K. (2020). Anti-tumor activities of the new oral pan-RAF inhibitor, TAK-580, used as monotherapy or in combination with novel agents in multiple myeloma. Oncotarget, 11(44), 3984-3997.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!