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Non targeting scramble sirna

Manufactured by Thermo Fisher Scientific
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Non-targeting scramble siRNA is a laboratory tool used in RNA interference (RNAi) experiments. It serves as a control to help assess the specificity of gene silencing effects observed when using other small interfering RNA (siRNA) molecules. This product does not target any known gene and is designed to have minimal sequence complementarity to the transcriptome.

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Lab products found in correlation

Lipofectamine rnaimax, by Thermo Fisher Scientific (6 mentions) Hbegf targeting sirna, by Santa Cruz Biotechnology (2 mentions)

Close spelling variants of this product

Non-targeting siRNA Scramble siRNA SiRNA targeting SiRNAs

3 protocols using non targeting scramble sirna

1

TFPI Knockdown in HUVECs

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The TFPI targeting siRNA was purchased from Santa Cruz (sc-41060). The non-targeting scramble siRNA was purchased from Life Technologies. HUVECs were seeded in 96-well plates for 24 h. When confluency reached 70%, cells were incubated in serum-free medium for 8 h. The siRNAs were transfected into cells using Lipofectamine RNAiMAX (ThermoFisher). Experiments were carried out 48 h later. The knockdown efficiency was validated by immunoblot analysis.
Tian S., Xiong X., Zeng J., Wang S., Tremblay B.J., Chen P., Chen B., Liu M., Chen P., Sheng K., Zeve D., Qi W., Breault D.T., Rodríguez C., Gerhard R., Jin R., Doxey A.C, & Dong M. (2022). Identification of TFPI as a receptor reveals recombination-driven receptor switching in Clostridioides difficile toxin B variants. Nature Communications, 13, 6786.
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2

siRNA-Mediated HBEGF Knockdown

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The HBEGF targeting siRNA was purchased from Santa Cruz (sc-39420). The non-targeting scramble siRNA was purchased from Life Technologies. Cells were seeded in 24-well plates for 24 h. When confluency reached 70%, cells were incubated in serum-free medium for 8 h. The siRNAs were transfected into cells using Lipofectamine RNAiMAX (ThermoFisher). Experiments were carried out 48 h later. The knockdown efficiency was validated by immunoblot analysis.
Tian S., Liu Y., Appleton E., Wang H., Church G.M, & Dong M. (2022). Targeted intracellular delivery of Cas13 and Cas9 nucleases using bacterial toxin-based platforms. Cell reports, 38(10), 110476.
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3

siRNA-Mediated HBEGF Knockdown

Check if the same lab product or an alternative is used in the 5 most similar protocols
The HBEGF targeting siRNA was purchased from Santa Cruz (sc-39420). The non-targeting scramble siRNA was purchased from Life Technologies. Cells were seeded in 24-well plates for 24 h. When confluency reached 70%, cells were incubated in serum-free medium for 8 h. The siRNAs were transfected into cells using Lipofectamine RNAiMAX (ThermoFisher). Experiments were carried out 48 h later. The knockdown efficiency was validated by immunoblot analysis.
Tian S., Liu Y., Appleton E., Wang H., Church G.M, & Dong M. (2022). Targeted intracellular delivery of Cas13 and Cas9 nucleases using bacterial toxin-based platforms. Cell reports, 38(10), 110476.
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