Epimedin b

Adenosine triphosphate (ATP), nigericin, SiO₂, poly (deoxyadenylic-thymidylic) acid sodium salt (poly (dA:dT)), polyinosinic: polycytidylic acid (poly (I:C)), Pam3CSK4, dimethyl sulfoxide (DMSO) and LPS (Escherichia coli, 055: B5) were purchased from Sigma-Aldrich (Munich, Germany). Epimedin A (110623–72-8, purity 99.0%), Epimedin A₁ (140147–77-9, purity 99.92%), epimedin B (110623–73-9, purity 99.39%), epimedin C (110642–44-9, purity 99.1%), icariin (489–32-7, purity 97.64%), icaritin (118525–40-9, purity 99%), Icariside I (56725–99-6, purity 99.47%) and anhydroicaritin (38226–86-7, purity 99.51%) were purchased from TargetMol. Salmonella was kindly provided by Dr. Tao Li from National Center of Biomedical Analysis. MCC950 was obtained from TargetMol (Boston, USA). Anti-mouse caspase-1(1:1000, AG-20B-0042) was purchased from Adipogen (San Diego, USA). anti-mouse IL-1β (1:1000, 12,507), and anti-NLRP3 (1:2000, 15101S) were obtained from Cell Signaling Technology (Boston, USA). Anti-ASC (1:1000, sc-22,514-R) was purchased from Santa Cruz Biotechnology (Dallas, USA). Anti-GAPDH (1:2000, 60,004–1-1 g) was purchased from Proteintech (Chicago, USA). Color Prestained Protein marker (20AB01) was purchased from GenStar (Beijing, China).

Adenosine triphosphate (ATP), Nigericin, SiO2, poly (deoxyadenylic-thymidylic) acid sodium salt (poly (dA:dT)), polyinosinic: polycytidylic acid [poly (I:C)], Pam3CSK4, dimethyl sulfoxide (DMSO), and LPS (Escherichia coli, 055: B5) were purchased from Sigma-Aldrich (Munich, Germany). Epimedin A (110623–72–8, purity 99.0%), Epimedin A1 (140147–77–9, purity 99.92%), epimedin B (110623–73–9, purity 99.39%), epimedin C (110642–44–9, purity 99.1%), icariin (489–32–7, purity 97.64%), caritin (118525–40–9, purity 99%), and anhydroicaritin (38226–86–7, purity 99.51%) were purchased from TargetMol. Salmonella strains were kindly provided by Dr. Tao Li from the National Center of Biomedical Analysis. MCC950 was obtained from TargetMol (Boston, MA, United States). Anti-mouse caspase-1(1:1000, AG-20B-0042) was purchased from Adipogen (San Diego, CA, United States). Anti-mouse IL-1β (1:1000, 12,507) and anti-NLRP3 (1:2000, 15101S) antibodies were obtained from Cell Signaling Technology (Boston, MA, United States). Anti-ASC (1:1000, sc-22,514-R) was purchased from Santa Cruz Biotechnology (Dallas, TX, United States). Anti-GAPDH (1:2000, 60,004–1-1 g) was purchased from Proteintech (Chicago, IL, United States). The color prestained protein marker (20AB01) was purchased from GenStar (Beijing, China).