The concentration of serum high sensitive (hs)CRP was determined by immunoturbidimetry. While SAA was measured in serum, HDL2 and HDL3 by a commercially available ELISA (Invitrogen, Human SAA KHA0011C) and the analysis was performed on a Grifols TRITURUS automated ELISA system (Italy), as per the manufacturer’s instructions, with the following modifications prior to analysis: serum was diluted 1:150, HDL2 1:10 and HDL3 1:100.
Triturus automated elisa system
Manufactured by Grifols
Sourced in Italy
The TRITURUS automated ELISA system is a laboratory instrument designed for performing enzyme-linked immunosorbent assays (ELISA) in a standardized and automated manner. The core function of the TRITURUS is to automate the various steps involved in ELISA testing, including sample and reagent handling, incubation, and signal detection. This system helps streamline ELISA workflows and improve consistency in results.
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Lab products found in correlation
3 protocols using triturus automated elisa system
1
Serum Biomarkers Determination
2
Serum SAA Quantification by ELISA
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SAA levels were measured in serum samples isolated from whole blood following centrifugation at 3000 rpm at 4 °C for 10 min using an enzyme-linked immunosorbent assay (ELISA, Invitrogen™ Human SAA kit KHA0011C, CA, USA) using a Grifols Triturus automated ELISA system (Vicopisano, Italy) as per the manufacturer's instructions. The coefficients of variation for SAA were 2.8% (interspecific) and 8.0% (intraspecific).
3
Quantifying Serum and HDL-Associated SAA
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The concentration of SAA was measured in serum, HDL2 and HDL3 by a commercially available ELISA procedure, which measures SAA-1 (Invitrogen, Human SAA KHA0011C). SAA was assessed on a Grifols TRITURUS automated ELISA system (Italy), as per the manufacturer's instructions, with the following modifications: Serum was diluted 1:150, HDL2 was diluted 1:10 and HDL3 diluted 1:100 prior to analysis. The intra-assay CVs for serum-, HDL2-and HDL3-SAA were 2.8, 3.7 and 5.6%, respectively, while their inter-assay CVs were 8, 9.5 and 11%, respectively. Non-protein standardised results are presented as µg/L, which is the absolute concentration in the serum or in the HDL2 and HDL3 subfractions after isolation. In addition, SAA associated with HDL2 and HDL3 was also standardised for protein concentration and is presented as μg SAA/mg protein.
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