Log In Sign up
The largest database of trusted experimental protocols

Amicon ultra 15 centrifugal filter device 100k

Manufactured by Merck Group
Visit Supplier

The Amicon Ultra-15 Centrifugal Filter Device 100K is a laboratory filtration device. It is used for the concentration and buffer exchange of macromolecules, such as proteins, in liquid samples.

Automatically generated - may contain errors

Lab products found in correlation

Synergy h4 hybrid microplate reader, by Agilent Technologies (1 mentions) Normal horse serum, by Vector Laboratories (1 mentions) Clodronate liposome, by Encapsula Nanosciences (1 mentions) Tissue tek, by Avantor (1 mentions) Fetal bovine serum (fbs), by Atlas Biologicals (1 mentions) Penicillin streptomycin solution, by Merck Group (1 mentions) Celltiter 96 aqueous non radioactive cell proliferation assay, by Promega (1 mentions) Vectashield antifade mounting medium with dapi, by Vector Laboratories (1 mentions) Dulbecco s modified eagle s medium, by Corning (1 mentions) Raw264.7 mouse macrophage cells, by American Type Culture Collection (1 mentions)

Close spelling variants of this product

Amicon 100K centrifugal filter Amicon Ultra-15 100K Amicon Ultra 100K Amicon centrifugal devices Amicon centrifugal filters Centrifugal filter devices Amicon filter devices Amicon Ultra-15 Amicon Ultra device Amicon Ultra filters

2 protocols using amicon ultra 15 centrifugal filter device 100k

1

Liposome Stability and Fluorophore Detachment

Check if the same lab product or an alternative is used in the 5 most similar protocols
Liposomes (25 μL) were incubated in Prigrow II Medium containing 10% FBS (0.9 mL) on a shaker at 37 °C. At various time points, 20 μL of the solution was removed and mixed with distilled water (980 μL). The size and PDI of the liposomes was determined by DLS as described in the nanoparticle section. For fluorophore detachment studies, liposomes (3 μL) were incubated in Prigrow II Medium containing 10% FBS (1.4 mL) on a shaker at 37 °C. Centrifugation (4,000 × g; 30 min) of the solution in Amicon Ultra-15 Centrifugal Filter Device 100K (Millipore Sigma) was performed to separate liposomes from detached fluorophores. Serial dilutions of the ultrafiltrate were placed in a black clear-bottom 96-well plate and assayed for fluorescence levels using a Synergy H4 Hybrid Microplate Reader (BioTek). The stability of 89Zr-labeled silicon particles (351 KBq/100 μL) was evaluated in saline containing 50% mouse serum (1 mL; 37 °C) using an EZ-SCAN TLC Scanner (Carroll & Ramsey Associates).
Wolfram J., Nizzero S., Liu H., Li F., Zhang G., Li Z., Shen H., Blanco E, & Ferrari M. (2017). A chloroquine-induced macrophage-preconditioning strategy for improved nanodelivery. Scientific Reports, 7, 13738.
+ Open protocol
+ Expand
2

Liposome-Mediated Macrophage Uptake Study

Check if the same lab product or an alternative is used in the 5 most similar protocols
Materials were acquired from the following sources: dioleoyl-phosphatidylcholine (DOPC)/cholesterol (CHOL) liposomes (F60103F-TR) and DOPC/CHOL/mPEG-distearoyl-phosphoethanolamine (DSPE) liposomes (F60203F-TR) labeled with Texas Red-dihexadecanoyl-phosphoethanolamine from FormuMax Scientific; clodronate liposomes from Encapsula NanoSciences; Prigrow II Medium and immortalized rat Kupffer cells from Applied Biological Materials; Raw 264.7 mouse macrophage cells from American Type Culture Collection (ATCC); fetal bovine serum (FBS) from Atlas Biologicals; 96-well flat clear bottom black polystyrene TC-treated microplates and Dulbecco’s Modified Eagle’s Medium (DMEM) with 4.5 g/L glucose, L-glutamine & sodium pyruvate from Corning; penicillin-streptomycin solution from Sigma-Aldrich; ethylendiaminetetraacetic acid (EDTA) and phosphate buffered saline (PBS; HyClone) from Thermo Fisher Scientific; F4/80: Alexa Fluor 647 antibody from Bio-Rad (MCA497A488); CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay from Promega; Microtainer Tubes with K2E from BD; Amicon Ultra-15 Centrifugal Filter Device 100K from Millipore Sigma; Tissue-Tek optimum cutting temperature (O.C.T) compound form VWR; Vectashield Antifade Mounting Medium with DAPI (4′,6-diamidino-2-phenylindole) and normal horse serum from Vector Laboratories.
Samuelsson E., Shen H., Blanco E., Ferrari M, & Wolfram J. (2017). Contribution of Kupffer cells to liposome accumulation in the liver. Colloids and surfaces. B, Biointerfaces, 158, 356-362.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!