Item imaging software

Manufactured by EMSIS

ITEM imaging software is a digital image analysis tool designed for scientific and medical applications. It provides a platform for capturing, processing, and analyzing digital images obtained from various imaging devices.

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Lab products found in correlation

Carbon coated 400 mesh copper grid, by Agar Scientific (1 mentions) Megaview g2, by EMSIS (1 mentions) Ultramicrotome, by Leica (1 mentions) Tecnai transmission electron microscope, by Olympus (1 mentions) Morada ccd camera, by EMSIS (1 mentions)

2 protocols using item imaging software

Phage Particle Visualization via TEM

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For TEM analysis phage particles produced from MmC-treated cultures (in G-M17 growth broth) were adsorbed to a carbon-coated 400-mesh copper grid (Agar Scientific, Essex, United Kingdom) and negative staining with 2% (w/v) uranyl acetate was performed as described previously (Deasy et al., 2011 (link)). Specimens were examined with a Tecnai 10 transmission electron microscope (FEI Thermo Fisher Scientific, Eindhoven, The Netherlands) operated at an acceleration voltage of 80 kV. Micrographs were taken with a MegaView G2 charge-coupled-device camera (EMSIS, Münster, Germany). All measurements of the phage particle dimensions were performed using iTEM imaging software (EMSIS).

Oliveira J., Mahony J., Hanemaaijer L., Kouwen T.R., Neve H., MacSharry J, & van Sinderen D. (2017). Detecting Lactococcus lactis Prophages by Mitomycin C-Mediated Induction Coupled to Flow Cytometry Analysis. Frontiers in Microbiology, 8, 1343.

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Transmission Electron Microscopy of Cultured Cells

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The cultured cells were fixed with 2.5% glutaraldehyde in 0.1 M cacodylate buffer (pH 7.4), then post-fixed in 1% OsO₄ in the same cacodylate buffer at room temperature for 1 hour. After staining en bloc with 2% aqueous uranyl acetate for 30 min, cells were dehydrated in a graded series of ethanol to 100% and finally embedded in EMbed 812 resin. Blocks were then polymerized in a 60°C oven for 24 hr. Thin sections (60 nm) were cut by a Leica ultramicrotome and post-stained with 2% uranyl acetate and lead citrate. Sample grids were examined with a FEI Tecnai transmission electron microscope at 80 kV of accelerating voltage, and digital images were recorded with an Olympus Morada CCD camera and iTEM imaging software (EMSIS GmbH).

Jesinkey S.R., Madiraju A.K., Alves T.C., Yarborough O.H., Cardone R.L., Zhao X., Parsaei Y., Nasiri A.R., Butrico G., Liu X., Molina A.J., Rountree A.M., Neal A.S., Wolf D.M., Sterpka J., Philbrick W.M., Sweet I.R., Shirihai O.H, & Kibbey R.G. (2019). Mitochondrial GTP Links Nutrient Sensing to β Cell Health, Mitochondrial Morphology, and Insulin Secretion Independent of OxPhos. Cell reports, 28(3), 759-772.e10.

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