The surface morphology of the liposomes was studied using scanning electron microscopy (Zeiss EVO LS10, Cambridge, UK).20 Samples were fixed on stubs using both side adhesive carbon tape (SPI Supplies, West Chester, PA, USA) and coated under vacuum with gold in a Q150R sputter coater unit obtained from Quorum Technologies Ltd. (East Sussex, UK) in an argon atmosphere at 20 mA for 120 seconds. After mounting, the samples were viewed and photographed. Particles size analyzer was used to determine the size of the liposomal entity (Mastersizer 2000, Malvern Instruments, Malvern, UK). Laser diffraction technique was applied to determine the liposomal size distribution by immersing liposomes in oil medium at a temperature of 25°C±1°C and scattering light at 90°.
Side adhesive carbon tape
Manufactured by SPI Supplies
Sourced in United States, United Kingdom
Side adhesive carbon tape is a specialized laboratory equipment used to hold or secure samples or materials during various experimental procedures. It features a strong adhesive backing made of carbon material, providing a secure and reliable attachment surface. The core function of this product is to aid in the manipulation and positioning of test specimens or apparatus within a laboratory setting.
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2 protocols using side adhesive carbon tape
1
Liposomal Surface Morphology Characterization
2
SEM Characterization of Drug Formulations
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The changes in the shape and surface morphology of the pure drugs and SD samples were evaluated using SEM. Photomicrographs of the samples were taken using SEM microscope (Zeiss EVO LS10; Cambridge, UK). The samples were fixed on stubs using both side adhesive carbon tape (SPI Supplies, West Chester, PA) and coated under vacuum with gold in a Q150R sputter coater unit from Quorum Technologies Ltd. (East Sussex, UK) in an argon atmosphere at 20 mA for 60 sec.
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