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2 protocols using pg 17 0 17 0

1

Lipid Extraction and Derivatization Protocol

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Saturated and unsaturated FFAs were obtained from Sigma-Aldrich. Phospholipid standards (PC 17:0/17:0, PE 17:0/17:0, PS 17:0/17:0, PA 17:0/17:0, PG 17:0/17:0, PC 12:0/13:0, PE 17:0/14:1, PS 17:0/14:1, PA 12:0/13:0 and PG 12:0/13:0) were purchased from Avanti Polar Lipids. 1,1-carbonydiimidiazole, 1,4 dioxane, triethylamine, iodomethane, iodomethane-d3, iodoethane, formic acid, citric acid, disodium hydrogen phosphate ammonium formate, acetonitrile, 1-butanol, methanol and chloroform were purchased from Sigma Aldrich. All reagents were analytical grade or equivalent. All lipid extractions were performed in 2 mL polypropylene LoBind safe-lock tubes (Eppendorf).
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2

Comprehensive Metabolite Profiling Approach

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High-performance liquid chromatography (HPLC)-grade ammonium acetate, acetonitrile, methanol, chloroform and water were procured from Burdick & Jackson (Morristown, NJ). MS grade formic acid, standards and internal standards, N-acetyl aspartic acid-d3, tryptophan-15N2, sarcosine-d3, glutamic acid-d5, thymine-d4, gibberellic acid, trans-zeatin, jasmonic acid, anthranilic acid-15N, and testosterone-d3, were purchased from Sigma-Aldrich (St. Louis, MO). Mixture of LPC 17:0/0:0, PG 17:0/17:0, PE 17:0/17:0, PC 17:0/17:0, TAG 17:0/17:0/17:0, SM 18:1/17:0, MAG 17:0, DAG 16:0/18:1, CE 17:0, ceramide 18:1/17:0, PA 17:0, PI 17:0/20:4, and PS 17:0/17:0. were obtained from Avanti polar lipids (USA) (Supplementary Table S1).
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