A two-centimeter divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/ PDMS) SPME fiber (purchased from Sigma Aldrich, St. Louis, MO, USA) was used to preconcentrate VOCs. Guanidine hydrochloride (GHCl; pH = 8.5) was purchased from Sigma Aldrich and used to denature major urinary proteins (MUPs), as they bind VOCs in hydrophobic pockets [35 (link)]. Headspace vials with a volume of 10 mL were purchased from Thermo Fisher Scientific (Waltham, MA, USA) and were used for urine sample storage and analysis. An Agilent (Santa Clara, CA, USA) 7890A GC system coupled to an Agilent 7200 MS quadrupole time-of-flight (QTOF) equipped with a PAL autosampling system (CTC Analytics, Zwingen, Switzerland) was used to incubate, extract, and analyze VOCs. The GC column utilized for VOC separation was an Agilent Ultra Inert HP-5 ms with 30 m length, 250 μm internal diameter, and 0.25 μm film thickness. MATLAB R2020a and Origin were used to generate figures.
Dvb car pdms spme fiber
Manufactured by Merck Group
Sourced in United States
The DVB/CAR/PDMS SPME fiber is a solid-phase microextraction (SPME) fiber designed for the extraction and preconcentration of a wide range of volatile and semi-volatile organic compounds. The fiber is composed of a combination of divinylbenzene (DVB), carboxen (CAR), and polydimethylsiloxane (PDMS) phases, which provide versatile extraction capabilities for a variety of analytes. This fiber is suitable for use in various analytical applications that require the isolation and concentration of target compounds prior to instrumental analysis.
Automatically generated - may contain errors
Lab products found in correlation
Stableflex, by Merck Group (2 mentions)
Ctc combipal, by CTC Analytics (2 mentions)
Pal autosampling system, by CTC Analytics (1 mentions)
7890a gc system, by Agilent Technologies (1 mentions)
Guanidine hydrochloride ghcl, by Merck Group (1 mentions)
Pegasus 3, by Leco (1 mentions)
Zb wax capillary column, by Phenomenex (1 mentions)
Odp 3, by Gerstel (1 mentions)
5890 series 2, by Hewlett-Packard (1 mentions)
17 protocols using dvb car pdms spme fiber
1
Volatile Organic Compounds Profiling in Urine
2
Volatile Organic Compound Extraction
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VOCs were extracted using a divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) SPME fiber (50/30 µm thickness), which was purchased from Sigma-Aldrich (Supelco, Bellefonte, PA, USA). Extraction of VOCs from samples was achieved with the AOC 6000 auto-sampler (Shimadazu, Tokyo, Japan). Before each analysis, fiber was conditioned at 250 °C for 10 min. For enhancing the dissociation of VOCs, samples were incubated at 80 °C for 15 min. SPME fiber was then inserted into the headspace of the vial for 15 min (agitator speed was 300 rpm) to extract VOCs at 80 °C.
3
HS-SPME Volatile Extraction in Dry-Cured Ham
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The SPME extraction procedure was performed using a Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS) SPME fiber (2 cm × 50/30 μm thickness) purchased from Sigma-Aldrich (Supelco, Bellefonte, USA). The SPME fiber was pre-conditioned at 270°C for 30 min before the first use. Before each extraction, the SPME fiber was conditioned for 5 min at 250°C before analysis and for 20 min at 250°C after analysis. For each sample, 1.0 g of the standard solution was weighed into a 10 mL SPME glass vial (Supelco), followed by 1 mL of a saturated NaCl solution and 50 μL of the ISTD mix. The same procedure was replicated for the dry-cured ham samples. The vials were tightly capped with a silicone/PTFE septum. The volatiles were then extracted under optimal HS-SPME conditions, which included a 60-min equilibration time and a 60-min extraction time, both at a temperature of 70°C. Afterwards, the analytes were desorbed for 4 min in the GC injector (250°C) equipped with a straight Ultra Inert Solid Phase Microextraction Liner (Sigma-Aldrich, Supelco, USA) operating in splitless mode. Each sample was analyzed in duplicate.
4
Volatile Compound Identification via SPME-GC-MS
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The identities of the volatile compounds were obtained using solid phase micro-extraction (SPME) and gas chromatography-mass spectrometry (GC-MS). The volatiles were collected from the sealed vials containing different TAG samples after incubation using a 50/30 μm DVB/CAR/PDMS SPME fiber (Supelco, Bellafonte, PA, USA). The SPME fiber was exposed to the headspace for 5 min at room temperature under the same conditions. The absorbed volatiles were then desorbed in the injection port of the gas chromatograph GC-2010 equipped with a Model GCMS-QP2010 Ultra mass spectrometer (Shimadzu Corporation). The GC conditions were the same as described above. The mass spectrometer was operated in the electron impact ionization mode (70 eV). The identification of the volatile compounds was performed by comparison with the mass spectra from the NIST Standard Reference Database and by injection of authentic standards. Acrolein peak was identified from parent ion (m/z 56) and other target ions (m/z 55 and 27). In addition to GC-MS analysis, authentic sample of acrolein was used for the identification of the GC peak. GC was carried out using two different columns, namely HP-1 and DB-35.
5
Headspace SPME-GC-MS Analysis of Volatile Compounds in Roasted Sunflower Seeds
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For extraction of volatiles, the ground roasted sunflower seeds (2 g) was placed into headspace extraction vial, with 100 μg/2 g ethyl decanoate of sunflower seed (ethyl decanoate 1 mg/ml in 10% methanol) internal standard, prior to sealing with caps. 1 μl C6-C26 n-alkanes mixture (100 μg/ml each in methanol) were analyzed under the same condition. The sample was equilibrated for 20 min at 60 °C in the HS of the vial. After the equilibration, a 50/30 μm DVB/CAR/PDMS SPME fiber (57348-U, Supelco) was exposed to the HS for 30 min at 60 °C. PDMS is used for non-polar analytes, DVB is for polar analytes, especially useful for pyrazines. The application of this fiber successfully identified the aroma compounds of roasted almond29 (link) and roasted plantains30 (link).
6
Extraction and Analysis of Tea Volatiles
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Extraction and analysis of the volatile compounds collected from the samples of the leaves of tea plantlets cultivated after14 d were performed with a headspace-solid phase microextraction (HS-SPME) fiber, coupled with gas chromatography (Agilent 7697A) and mass spectrometry (Agilent 7890A) (GC/MS). In brief, 0.3 g of leaves samples were cut up and put in the 20 ml headspace bottle 4 mL by adding boiling double distilled water dissolved 0.8 g KCl. After incubation for 1.5 min, the volatile compounds were collected using a 50/30 μm DVB/CAR/PDMS SPME fiber (Supelco, PA, USA) for 50 min at 70 °C and then desorbed into the GC injection port at 250 °C for 5 min. Subsequently, the volatile compounds were resolved by BD-5 capillary column (30 m × 0.25 mm × 0.25 μm, Agilent) for GC/MS analysis according to Han et al. [64 (link)].
7
Volatile Compound Analysis of Soybean Paste
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The volatile flavor compounds in soybean paste samples were measured by GC–MS as described by Jeong et al. (2019 (link)). Soybean paste sample (3.0 g) was mixed with 10 μL cyclohexanone [10 μg/mL (w/v) in ethanol] as an internal standard in 20 mL SPME vials. The sample bottle was put in a water bath at 65°C for 30 min, and the volatiles were absorbed by the 50/30 μm DVB/CAR/PDMS SPME fiber (Supelco). Volatile compounds were analyzed using a gas chromatograph (7890A) equipped with a mass selective detector (5975C) (Agilent). Helium as a carrier gas at a flow rate of 1.0 mL/min. The oven temperature procedure was as follows: 35°C for 3 min, 3°C/min to 50°C, 6–150°C, 10–230°C, and 230°C for 6 min. The other conditions were a collection mass range of 40–350 m/z, an ion source temperature of 230°C, and an electronic ionization (EI) mode of 70 eV. Volatile fractions were searched in NIST. Quantitative analysis was performed according to the concentration of the internal standard and the ratio of the peak areas of each component in the sample to the peak areas of the internal standard.
8
Solid Phase Micro-Extraction of Volatile Compounds
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The solid phase micro-extraction (SPME) fiber was used after pre-conditioning at
270°C for 30 min according to the manufacturer’s manual before
use. Briefly, 2.5 g of the minced sample was placed in a 20 mL screw-capped vial
and sealed with a screw cap fitted with polytetrafluroethylene (PTFE) septa. The
adsorption of volatiles was carried out for 55 min in an incubator at
45°C using 50/30 μm DVB/CAR/PDMS SPME fiber (57328-U, Supelco,
Bellefonte, PA, USA). After adsorption, the SPME fiber was immediately injected
into the GC inlet and desorbed for 3 min in pressure splitless mode (30 psi). At
this time, the GC inlet temperature was 240°C. Before adsorbing the next
sample, pre-conditioning was performed for 8 min while flowing carrier gas
(Helium, 99.999%) at 240°C at 100 mL/min for overall SPME fiber
cleaning.
270°C for 30 min according to the manufacturer’s manual before
use. Briefly, 2.5 g of the minced sample was placed in a 20 mL screw-capped vial
and sealed with a screw cap fitted with polytetrafluroethylene (PTFE) septa. The
adsorption of volatiles was carried out for 55 min in an incubator at
45°C using 50/30 μm DVB/CAR/PDMS SPME fiber (57328-U, Supelco,
Bellefonte, PA, USA). After adsorption, the SPME fiber was immediately injected
into the GC inlet and desorbed for 3 min in pressure splitless mode (30 psi). At
this time, the GC inlet temperature was 240°C. Before adsorbing the next
sample, pre-conditioning was performed for 8 min while flowing carrier gas
(Helium, 99.999%) at 240°C at 100 mL/min for overall SPME fiber
cleaning.
9
Volatile Compound Extraction from CSS
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The volatile compounds in CSS were also extracted by means of SPME, as described previously with some modifications [11 (link)]. A 2-cm (coated with 50/30 μm DVB/CAR/PDMS) SPME fiber (Supelco, Bellefonte, PA, USA) was preconditioned before extraction experiments in accordance with the manufacturer’s instructions. A mixture of 16 mL CSS and 2 g sodium chloride was placed in a 40-mL static headspace amber glass bottle fitted with a stir bar and a polytetrafluoroethylene (PTFE)-faced silicon septum. The extraction conditions for SPME obtained by optimizing experiments were as follows: equilibrium and extraction temperatures of 45 °C, an equilibrium time of 20 min, and an extraction time of 40 min. After the extraction experiment, the fiber was transferred to the injector port of GC for a 5-min desorption at 250 °C to conduct the GC-O and GC-MS analyses.
10
Headspace GC-MS Analysis of Fecal Metabolites
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Whole fecal pellets were extracted in a 10 mL sample vial containing NaCl (0.4 g), deionized water (965 µL), 5 ppm aqueous d-11 hexanoic acid (10 µL), and 3N H2SO4 (15 µL) to acidify the sample solution to pH 2. Headspace solid phase microextraction (HS-SPME) sampling was automated by a CombiPal autosampler (LEAP Technologies, Carrboro, NC, USA) by agitating the samples at 500 rpm (5 s on and 2 s off) during a 30 min equilibration at 40 °C. Volatile compounds were extracted from the headspace with a 1 cm 50/30 µm DVB/CAR/PDMS SPME fiber (Supelco, Bellefonte, PA, USA) for 30 min at 40 °C before thermal desorption into the gas chromatography inlet. Nontargeted GCxGC-ToFMS was performed on an LECO Pegasus III coupled with an Agilent GC retrofitted with a secondary oven and cryogenic modulator (Leco Corporation, St. Joseph, MI, USA). The detector voltage was optimized by ChromaTOF between −1418.0 and −1472.2 V, and masses of 25–500 were collected at a rate of 500 spectra per second, as described previously [18 (link),19 (link)]. The abundance of each metabolite was expressed as the log2-transformed average change in the metabolite peak area from that of the HFD controls.
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