Puregene whole blood dna extraction kit

Manufactured by Qiagen

Sourced in United States

The Puregene whole-blood DNA extraction kit is a laboratory product designed for the purification of genomic DNA from whole blood samples. It provides a reliable and efficient method for extracting high-quality DNA for various downstream applications.

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Lab products found in correlation

Iplex technology, by Labcorp (1 mentions) Light cycler 480 genotyping master, by Roche (1 mentions)

Close spelling variants of this product

DNA extraction kit (652 citations) Puregene kit (161 citations) DNA blood kit (56 citations) Puregene Blood Kit (51 citations) Blood DNA extraction kit (35 citations) Puregene extraction kit (18 citations) DNA kits (7 citations) Whole blood (4 citations) Blood kits (3 citations)

2 protocols using puregene whole blood dna extraction kit

DNA Extraction and SNP Analysis

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DNA was extracted from 10 mL of ethylenediaminetetraacetic acid (EDTA) blood using the Puregene whole-blood DNA extraction kit (Gentra Systems, Minneapolis, MN, USA). FKBP5, NR3C1, and CRHR1 SNPs were analysed on a Sequenom platform using the iPlex technology (Sequenom, San Diego, CA, USA) in a multiplex assay using 10 ng of DNA. For quality control, duplicate DNAs as well as negative controls were included in the genotyping plates. Genotype calls were made using the ArrayTyper 3.4 software (Sequenom, San Diego, CA, USA).

Schneider M., Engel A., Fasching P.A., Häberle L., Binder E.B., Voigt F., Grimm J., Faschingbauer F., Eichler A., Dammer U., Rebhan D., Amann M., Raabe E., Goecke T.W., Quast C., Beckmann M.W., Kornhuber J., Seifert A, & Burghaus S. (2014). Genetic Variants in the Genes of the Stress Hormone Signalling Pathway and Depressive Symptoms during and after Pregnancy. BioMed Research International, 2014, 469278.

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FKBP5 SNP rs1360780 Genotyping

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Blood samples (7.5mL) were collected in ethylenediaminetetraacetic acid containing tubes at baseline and DNA was extracted using the Puregene whole blood DNA-extraction kit (Gentra Systems Inc.). The FKBP5 SNP rs1360780 was genotyped by using real-time PCR and subsequent melting curve analysis performed with a Lightcycler 480 Genotyping Master (Roche Applied Science, Mannheim, Germany). The minor allele frequency was 28%. Genotype distribution did not deviate from the Hardy-Weinberg equilibrium as indicated by a nonsignificant P value of the exact test (P=.110). The SNP could be successfully genotyped in all samples (call rate=100%).

Höhne N., Poidinger M., Merz F., Pfister H., Brückl T., Zimmermann P., Uhr M., Holsboer F, & Ising M. (2015). FKBP5 Genotype-Dependent DNA Methylation and mRNA Regulation After Psychosocial Stress in Remitted Depression and Healthy Controls. International Journal of Neuropsychopharmacology, 18(4), pyu087.

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